本研究分析了中国南方和北方的石油污染土壤,以探索其结构,多样性,微生物群落的功能基因和组装过程。与南部地区的土壤相比,北部地区的土壤pH值升高,总氮(TN),和总石油烃(TPH)水平。其中,TN和TPH对微生物群落影响最大。细菌的优势门,古细菌,真菌是变形杆菌,Thaumarcheota,和Ascomycota,分别。其中,变形杆菌与各种功能基因密切相关,包括alkB和许多芳烃降解和反硝化基因(r>0.9,p<0.01)。表明变形杆菌在石油污染的土壤中起着重要作用。北部地区的新陈代谢比南部地区活跃。北部地区呈现明显的反硝化趋势,而南部地区的特征是乙酸碎屑甲烷生成。微生物群落的组装表现出区域模式,确定性组装在北方土壤中更为突出,而随机组装在南部土壤中很明显。总的来说,这些发现为理解石油污染土壤中的生物圈提供了一个新的概念框架,可能指导改善环境修复管理实践。
This study analyzed petroleum-contaminated soils from south and north locations in China to explore the structure, diversity, functional genes and assembly processes of microbial communities\' . Compared with soils from south locations, soils from northern regions exhibited elevated pH, total nitrogen (TN), and total petroleum hydrocarbon (TPH) levels. Among these, TN and TPH were the most influential on the microbial community. The dominant phyla for bacteria, archaea, and fungi were Proteobacteria, Thaumarchaeota, and Ascomycota, respectively. Among them, Proteobacteria was strongly correlated with various functional genes including alkB and many aromatics degradation and denitrification genes (r > 0.9, p < 0.01), suggesting that Proteobacteria play an important role in petroleum-contaminated soils. Metabolism in northern regions was more active than that in southern regions. The northern regions showed a pronounced tendency for denitrification, while the southern regions were characterized by acetoclastic methanogenesis. The assembly of microbial communities exhibited regional patterns, the deterministic assembly was more prominent in the northern soils, while the stochastic assembly was evident in the southern soils. Overall, these findings provide a new conceptual framework to understand the biosphere in petroleum-contaminated soil, potentially guiding improved management practices in the environmental remediation.