Abstract：
Synthesis of metal nanoparticles using plant extracts is environmentally friendly and of increasing interest. However, not all plant extracts can meet successfully on the synthesis. Therefore, searching for the high potential extracts that can reduce the metal salt precursor in the synthesis reaction is essential. The present study explores the synthesis of copper oxide nanoparticles (CuONPs) using Caesalpinia sappan heartwood extract. Phytochemical analysis and determination of the total phenolic content of the extract were performed before use as a reducing agent. Under the suitable synthesized condition, a color change in the color of the solutions to brown confirmed the formation of CuONPs. The obtained CuONPs were confirmed using ultraviolet-visible spectroscopy, photon correlation spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive X-ray, and Fourier transform infrared analysis. The synthesized CuONPs investigated for antioxidant, antiglycation, and antibacterial activities. CuONPs possessed antioxidant activities by quenching free radicals with an IC50 value of 63.35 µg/mL and reducing activity with an EC range of 3.19-10.27 mM/mg. CuONPs also inhibited the formation of advanced glycation end products in the bovine serum albumin/ribose model with an IC50 value of 17.05 µg/mL. In addition, CuONPs showed inhibition of human pathogens, including Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, and prevention of biofilm formation and biofilm eradication, with maximum inhibition of approx. 75%. Our findings suggest that C. sappan extract can be used to obtain highly bioactive CuONPs for the development of certain medical devices and therapeutic agents.
摘要：
使用植物提取物合成金属纳米颗粒是环境友好的并且越来越感兴趣。然而,并非所有的植物提取物都能在合成上成功相遇。因此,寻找能还原合成反应中金属盐前驱体的高电位萃取物至关重要。本研究探讨了使用Caesalpiniasappan心材提取物合成氧化铜纳米颗粒（CuONPs）。在用作还原剂之前进行植物化学分析和提取物总酚含量的测定。在合适的合成条件下,溶液颜色向棕色的颜色变化证实了CuONPs的形成。获得的CuONPs使用紫外-可见光谱进行确认,光子相关光谱学,X射线衍射,扫描电子显微镜，能量色散X射线，和傅里叶变换红外分析。合成的CuONPs研究了抗氧化剂，抗糖基化,和抗菌活性。CuONPs通过猝灭自由基而具有抗氧化活性，IC50值为63.35µg/mL，还原活性的EC范围为3.19-10.27mM/mg。CuONPs还抑制了牛血清白蛋白/核糖模型中晚期糖基化终产物的形成，IC50值为17.05µg/mL。此外,CuONPs显示抑制人类病原体，包括革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌，以及防止生物膜形成和生物膜根除，最大抑制约。75%。我们的发现表明C.sappan提取物可用于获得高度生物活性的CuONPs，用于开发某些医疗设备和治疗剂。
