背景:百果菌(较少。)DC。是一种原产于巴西的植物,经常在传统医学中用作利尿剂和抗高血压药。然而,尽管它传统上用于这些目的,其利尿和降压作用尚未完全阐明。
目的:研究百菌醇溶成分(EBM)对正常血压大鼠的心肾作用。
方法:使用光学和扫描电子显微镜分析了B.milleflora的Cladode,以提供解剖学数据来支持质量控制。随后,获得ESBM并使用LC-DAD-MS进行分析,并对其组件进行了注释。在雌性Wistar大鼠中评估了EBM的急性毒性。然后在Wistar大鼠中研究了急性和长期的利尿和降压作用。最后,我们评估了ESBM利尿作用的机制,包括肾Na+/K+/ATP酶的活性,血管紧张素转换酶,和红细胞碳酸酐酶.此外,我们还调查了缓激肽的参与,前列腺素,和一氧化氮。
结果:根据LC-DAD-MS数据,从ESBM中鉴定出33种代谢物,包括绿原酸,糖基化酚类衍生物,C-糖基化黄酮,和O-糖基化黄酮醇。在雌性大鼠中未观察到急性毒性的迹象。研究结果表明,ESBM具有显著的利尿和利尿钠作用,以及保钾效果。ESBM治疗能够显着降低血清肌酐和丙二醛水平,也显著增加了亚硝酸盐的含量,一氧化氮生物利用度的间接标志。此外,用L-NAME预处理可消除ESMM诱导的所有利尿作用。
结论:本研究提供了重要的形态解剖和植物化学数据,支持百菌的质量控制。在Wistar大鼠中,急性和7天重复治疗后,EBM表现出明显的利尿和利尿钠作用,不影响肾脏钾的消除。这些作用似乎取决于一氧化氮-环GMP途径的激活。这项研究表明,在需要利尿作用的临床情况下,水菌制剂的潜在用途。
BACKGROUND: Baccharis milleflora (Less.) DC. is a plant native to Brazil that is frequently used in traditional medicine as a diuretic and
antihypertensive. However, even though it is traditionally used for these purposes, its diuretic and hypotensive effects have not been fully elucidated.
OBJECTIVE: Investigate the cardiorenal effects of the ethanol-soluble fraction (ESBM) of Baccharis milleflora in normotensive rats.
METHODS: Cladodes of B. milleflora were analyzed using light and scanning electron microscopy to provide anatomical data to support quality control. Subsequently, the ESBM was obtained and analyzed using LC-DAD-MS, and its components were annotated. The acute toxicity of ESBM was assessed in female Wistar rats. The acute and prolonged diuretic and hypotensive effects were then studied in Wistar rats. Finally, we assessed the mechanisms responsible for the diuretic effects of ESBM, including the activity of renal Na+/K+/ATPase, angiotensin-converting enzyme, and erythrocyte carbonic anhydrase. Additionally, we also investigated the involvement of bradykinin, prostaglandins, and nitric oxide.
RESULTS: From LC-DAD-MS data, thirty-three metabolites were identified from ESBM, including chlorogenic acids, glycosylated phenolic derivatives, C-glycosylated flavones, and O-glycosylated flavonols. No signs of acute toxicity were observed in female rats. The findings showed that ESBM had significant diuretic and natriuretic effects, as well as a potassium-sparing effect. The treatment with ESBM was able to significantly decrease serum levels of creatinine and malondialdehyde, and also significantly increase levels of nitrite, an indirect marker of nitric oxide bioavailability. Furthermore, pre-treatment with L-NAME abolished all diuretic effects induced by ESBM.
CONCLUSIONS: This study presented important morpho-anatomical and phytochemical data that support the quality control of Baccharis milleflora. The ESBM exhibited a significant diuretic and natriuretic effect following acute and seven-days repeated treatment in Wistar rats, without affecting renal potassium elimination. These effects appear to be dependent on the activation of the nitric oxide-cyclic GMP pathway. This study suggests the potential use of B. milleflora preparations in clinical situations where a diuretic effect is needed.