PDF(Pubmed)
Abstract：
The Jatropha curcas cake, a protein-rich by-product of biofuel production, was the subject of our study. We identified and quantified the ACE inhibitory, antioxidant, and antidiabetic activities of bioactive peptides from a Jatropha curcas L. var Sevangel protein isolate. The protein isolate (20.44% recovered dry matter, 38.75% protein content, and 34.98% protein yield) was subjected to two enzyme systems for hydrolysis: alcalase (PEJA) and flavourzyme (PEJF), recording every 2 h until 8 h had passed. The highest proteolytic capacity in PEJA was reached at 2 h (4041.38 ± 50.89), while in PEJF, it was reached at 6 h (3435.16 ± 59.31). Gel electrophoresis of the PEJA and PEJF samples showed bands corresponding to peptides smaller than 10 kDa in both systems studied. The highest values for the antioxidant capacity (DPPH) were obtained at 4 h for PEJA (56.17 ± 1.14), while they were obtained at 6 h for PEJF (26.64 ± 0.52). The highest values for the antihypertensive capacity were recorded at 6 h (86.46 ± 1.85) in PEJF. The highest antidiabetic capacity obtained for PEJA and PEJF was observed at 6 h, 68.86 ± 8.27 and 52.75 ± 2.23, respectively. This is the first report of their antidiabetic activity. Notably, alcalase hydrolysate outperformed flavourzyme hydrolysate and the cereals reported in other studies, confirming its better multi-bioactivity.
摘要：
麻疯树蛋糕,一种富含蛋白质的生物燃料生产副产品，是我们研究的主题。我们鉴定并定量了ACE抑制，抗氧化剂，麻疯树varSevangel蛋白分离物的生物活性肽和抗糖尿病活性。分离蛋白(20.44%回收干物质,蛋白质含量38.75%,和34.98％的蛋白质产率）经过两种酶系统水解：碱性蛋白酶（PEJA）和风味酶（PEJF），每2小时记录一次，直到8小时过去。在PEJA中最高的蛋白水解能力在2小时达到（4041.38±50.89），而在PEJF,6h时达到(3435.16±59.31)。PEJA和PEJF样品的凝胶电泳显示在所研究的两种系统中对应于小于10kDa的肽的条带。PEJA在4小时时获得了抗氧化能力（DPPH）的最高值（56.17±1.14），而PEJF在6小时获得(26.64±0.52)。在PEJF中，抗高血压能力的最高值记录在6小时（86.46±1.85）。在6小时观察到PEJA和PEJF获得的最高抗糖尿病能力，分别为68.86±8.27和52.75±2.23。这是它们抗糖尿病活性的第一份报告。值得注意的是,alcalase水解产物优于风味酶水解产物和其他研究中报道的谷物，证实其更好的多生物活性。
