在目前的研究中,研究了丝树(Albiziakalkora)有机提取物的抗真菌生物活性潜力。kalkora和甲醇的粗提物,正己烷,氯仿,和制备乙酸乙酯级分。在0.39-50µg/mL的不同浓度下研究了获得的A.kalkora级分的抗真菌活性。二甲基亚砜(DMSO)作为毒性对照,而甲基托布津(TM)作为阳性对照。所有馏分均显着降低了FOL生长(甲醇:9.49-94.93%,正己烷:11.12-100%,氯仿:20.96-91.41%,和乙酸乙酯:18.75-96.70%)。正己烷级分显示6.25μg/mL的MIC,与具有64μg/mL的MIC的TM相比。与氯仿相比,非极性(正己烷)部分对FOL显示出最大的抗真菌生物活性,甲醇,和乙酸乙酯馏分。GC/MS分析表明,正己烷馏分中含有十六烷酸,9,12,15-十八碳三烯酸,9,12-十八碳二烯酸,邻苯二甲酸二(2-乙基己基)酯,硬脂酸甲酯,和[1,2,4]三唑并[1,5-a]嘧啶-6-甲酸。通过分子对接分析进一步增强了体外抗真菌抑制的结果。FOL的五种毒力蛋白,即,pH响应PacC转录因子(PACC),MeaB,TOR;雷帕霉素靶蛋白(FMK1),信号转导MAP激酶激酶(STE-STE7),通过GC/MS分析,将高渗透压甘油1(HOG1)与正己烷级分中鉴定的植物化合物对接。MEAB显示与锌酰亚胺的最大结合亲和力(-12.03kcal/mol),HOG1和FMK1分别带有α-Tocospiro-B(-11.51kcal/mol)和(-10.55kcal/mol),STE-STE7与二十二烷酸(-11.31kcal/mol),和PACC与十七烷酸(-9.88kcal/mol)分别具有与活性口袋残基的强疏水或亲水相互作用。总之,A.kalkora的正己烷部分可用于管理FOL。
The
antifungal bioactivity potential of the organic extract of silk tree (Albizia kalkora) was investigated in the current study. The crude extracts of A. kalkora and methanol, n-hexane, chloroform, and ethyl acetate fractions were prepared. The
antifungal activity of obtained fractions of A. kalkora was studied at different concentrations ranging from 0.39-50 µg/mL. Dimethyl sulfoxide (DMSO) was taken as a toxicity control, whereas thiophanate methyl (TM) as a positive control. All the fractions significantly reduced the FOL growth (methanolic: 9.49-94.93 %, n-hexane: 11.12-100 %, chloroform: 20.96-91.41 %, and ethyl acetate: 18.75-96.70 %). The n-hexane fraction showed 6.25 µg/mL MIC as compared to TM with 64 µg/mL MIC. The non-polar (n-hexane) fraction showed maximum
antifungal bioactivity against FOL in comparison with chloroform, methanol, and ethyl acetate fractions. GC/MS analysis exhibited that the n-hexane fraction contained hexadecanoic acid, 9,12,15-octadecatrienoic acid, 9,12-octadecadienoic acid, bis(2-ethylhexyl) phthalate, methyl stearate, and [1,2,4]triazolo[1,5-a]pyrimidine-6-carboxylic acid. The results of in vitro
antifungal inhibition were further reinforced by molecular docking analysis. Five virulence proteins of FOL i.e., pH-responsive PacC transcription factor (PACC), MeaB, TOR; target of rapamycin (FMK1), Signal transducing MAP kinase kinase (STE-STE7), and High Osmolarity Glycerol 1(HOG1) were docked with identified phytocompounds in the n-hexane fraction by GC/MS analysis. MEAB showed maximum binding affinities with zinnimide (-12.03 kcal/mol), HOG1 and FMK1with α-Tocospiro-B (-11.51 kcal/mol) and (-10.55 kcal/mol) respectively, STE-STE7 with docosanoic acid (-11.31 kcal/mol), and PACC with heptadecanoic acid (-9.88 kcal/mol) respectively with strong hydrophobic or hydrophilic interactions with active pocket residues. In conclusion, the n-hexane fraction of the A. kalkora can be used to manage FOL.