众所周知,具有显著性能的金属纳米粒子在比色检测过程中具有广阔的应用前景,催化作用,疾病诊断和治疗,能源,废水处理,补救,和抗菌活性。在这里,铁基纳米粒子(FeNPs),金属纳米颗粒,是通过使用超累积植物的简单化学还原法合成的。此外,研究了它们在抗菌活性应用和比色抗坏血酸(AA)检测中的应用。据观察,FeNPs对单核细胞增生李斯特菌和金黄色葡萄球菌的革兰氏阳性菌以及大肠杆菌的革兰氏阴性菌(O157:H7)具有很高的抗菌效力,大肠杆菌(ATCC25922),肠炎沙门氏菌,和鼠伤寒沙门氏菌.此外,发现FeNPs表现出优异的过氧化物酶样活性来催化3,3'的氧化,5,5'-四甲基联苯胺(TMB)生产蓝色产品,氧化TMB(oxTMB),在H2O2的存在下。由于AA的抗氧化性能,可以通过使溶液颜色变浅来进行比色AA检测。AA的定量检测可以简单地进行,有选择地,并且在30-200μM的线性范围内对检测限(LOD)为0.5462μM的FeNPs敏感。
It has been well known that metallic nanoparticles with striking properties possess wide application prospects in the processes of colorimetric detection, catalysis, disease diagnosis and treatment, energy, wastewater treatment, remediation, and antibacterial activity in recent years. Herein, iron-based nanoparticles (FeNPs), metallic nanoparticles, were synthesized via a facile chemical reduction method using a hyperaccumulator plant. Also, their use in antibacterial activity applications and colorimetric ascorbic acid (AA) detection was investigated. It was observed that FeNPs presented high antibacterial potency against Gram-positive bacteria of Listeria monocytogenes and Staphylococcus aureus and also Gram-negative bacteria of Escherichia coli(O157: H7), E. coli(ATCC 25922), Salmonella enteritidis, and Salmonella typhimurium. Moreover, it was found that FeNPs exhibited superior peroxidase-like activity to catalyze the oxidation of 3,3\',5,5\'-tetramethylbenzidine (TMB) to produce a blue color product, oxidized TMB (oxTMB), in the presence of H2O2. The colorimetric AA detection could be carried out by making the solution color lighter owing to the antioxidant property of AA. The quantitative detection of AA could be performed simply, selectively, and sensitively with FeNPs with a detection limit (LOD) of 0.5462 μM in a linear range of 30-200 μM.