石榴(Punicagranatum)是Punicaceae家族的一种树,遍布世界各地,具有多种类型和治疗用途。本研究旨在通过GC分析研究石榴籽油的植物化学成分,并对石榴籽油及其自纳米乳化体系进行物理表征。然后是抗氧化剂,抗糖尿病,并对两者的抗脂肪酶活性进行了研究。提取石榴籽油,制备了自纳米乳化体系。植物化学化合物通过GC分析,并建立了石榴籽油及其自纳米乳化体系的物理表征。然后是抗氧化剂,抗糖尿病,并对两者的抗脂肪酶活性进行了研究。GC-MS分析显示石榴酸,β-松脂酸,过氧化氢酸,α-松脂酸,和油酸是石榴籽油中最主要的化合物。其他活性化合物,如亚油酸,棕榈酸,硬脂酸,和α-亚麻酸以痕量百分比检测。使用各种浓度的表面活性剂(吐温80)制备自纳米乳化体系,助表面活性剂(Span80),还有石榴籽油.所选择的制剂具有0.229±0.09的PDI和189.44±2.1nm的液滴尺寸。石榴籽油的自由基清除活性,自乳化系统,使用DPPH进行Trolox。与Trolox相比,油自纳米乳化系统显示出有效的抗氧化活性。此外,石榴油抑制α-淀粉酶,IC50值为354.81±2.3µg/ml。与阿卡波糖相比,油自纳米乳化系统显示出有效的活性,并且与奥利司他相比具有较弱的IC50值(616.59±2.1µg/ml)和有效的IC50值(43.65±1.9µg/ml)。石榴籽油自纳米乳化系统可用于制备可能的预防和治疗氧化应激的口服药物。糖尿病,和肥胖,因为它对自由基的高活性,淀粉酶,和脂肪酶酶与石榴籽油本身和使用的参考文献进行比较。这项研究表明,自纳米乳液系统可以通过改善药代动力学和药效学来增强油药物制剂,作为药物储库,并促进有效的油释放。
Pomegranate (Punica granatum) is a tree of the Punicaceae family that is widespread all over the world and has several types and therapeutic uses. The current study aimed to investigate the phytochemical compounds by GC analysis and carried out physical characterization of the pomegranate seed oil and its self-nanoemulsifying system. Then antioxidant, anti-diabetic, and anti-lipase activities were investigated for both.The pomegranate seed oil was extracted, and its self-nanoemulsifying system was then prepared. Phytochemical compounds were analyzed by GC, and physical characterization was established of the pomegranate seed oil and its self-nanoemulsifying system. Then antioxidant, anti-diabetic, and anti-lipase activities were investigated for both.The GC-MS analysis revealed that punicic acid, β-eleosteric acid, catalpic acid, α-eleosteric acid, and oleic acid were the most predominant compounds in pomegranate seed oil. Other active compounds like linoleic acid, palmitic acid, stearic acid, and α-linolenic acid were detected in trace percentages. The self-nanoemulsifying system was prepared using various concentrations of surfactant (Tween 80), co-surfactant (Span 80), and pomegranate seed oil. The selected formulation had a PDI of 0.229 ± 0.09 and a droplet size of 189.44 ± 2.1 nm. The free radical scavenging activity of pomegranate seed oil, the self-emulsifying system, and Trolox was conducted using DPPH. The oil-self-nanoemulsifying system showed potent antioxidant activity compared to Trolox. Also, pomegranate oil inhibited α-amylase with a weak IC50 value of 354.81 ± 2.3 µg/ml. The oil self-nanoemulsifying system showed potent activity compared to acarbose and had a weaker IC50 value (616.59 ± 2.1 µg/ml) and a potent IC50 value (43.65 ± 1.9 µg/ml) compared to orlistat.Pomegranate seed oil self-nanoemulsifying system could be applied in the future for the preparation of possible oral medications for the prevention and treatment of oxidative stress, diabetes, and obesity due to its high activity against free radical, amylase, and lipase enzymes compared to pomegranate seed oil itself and the references used. This study reveals that self-nanoemulsion systems can enhance oil drug formulations by improving pharmacokinetics and pharmacodynamics, acting as drug reservoirs, and facilitating efficient oil release.