Abstract：
Piperlongumine (PLM), a natural compound isolated from long peppers, has been reported to possess multiple pharmacological roles, including anti-tumor and anti-diabetic. However, the pharmacological role of PLM on adipogenesis is still unknown. In this study, we found that PLM strongly inhibited 3T3-L1 adipocyte differentiation. This inhibition was determined by the accumulation of lipid droplets and intracellular triglycerides. In addition, PLM downregulated both the mRNA and protein expression of adipogenic transcription factors, including CCAAT-enhancer binding proteins β (C/EBPβ), C/EBPα, and peroxisome proliferator-activated receptor γ (PPARγ). Based on the time-course experiment, we found that the inhibitory effect of PLM on adipogenesis was mainly involved in the early stage of adipogenesis. Studying these differential effects could uncover new mechanisms for regulating adipogenesis and new chemicals for treating obesity.
摘要：
胡椒长胺(PLM),一种从长辣椒中分离出来的天然化合物，据报道具有多种药理作用，包括抗肿瘤和抗糖尿病。然而,PLM对脂肪生成的药理作用尚不清楚.在这项研究中,我们发现PLM强烈抑制3T3-L1脂肪细胞分化.这种抑制是通过脂滴和细胞内甘油三酯的积累来确定的。此外,PLM下调成脂转录因子的mRNA和蛋白表达,包括CCAAT增强子结合蛋白β（C/EBPβ），C/EBPα,和过氧化物酶体增殖物激活受体γ（PPARγ）。基于时程实验，我们发现PLM对脂肪生成的抑制作用主要在脂肪生成的早期阶段。研究这些不同的作用可能会发现调节脂肪生成的新机制和治疗肥胖的新化学物质。
