背景:牙髓封闭剂在根管间隙闭塞中起着至关重要的作用。这项研究的目的是评估最近开发的聚氨酯可膨胀密封剂(PES)的实用性,随着其细胞毒性和尺寸的变化。
方法:使用L929成纤维细胞和细胞活力测定(MTS测定)来确定牙齿密封剂的细胞毒性(AHPlus[DentsplyMaillefer,Ballaigues,瑞士],Sure-SealRoot[SureDentCorporation,京夷道,韩国],和PES)在24、48、72和96小时。使用高级脉络膜新生血管化模型来评估这些封闭剂对血管生成的影响。制备36颗拔除的单根人牙,随机分为3组(n=12)。使用牙胶胶和密封剂使用侧向压实进行填充,如下所示:第1组,AHPlus;第2组,Sure-Seal;和第3组,PES。用扫描电子显微镜测量密封剂渗透到牙本质小管中的平均深度。使用单向方差分析和事后Tukey检验(显著性水平,P<0.05)。
结果:MTS的值,脉络膜新生血管,PES的穿透深度明显高于其他实验组(P<0.05)。在AHPlus的标本中发现了最低值,而在PES组中检测到最高。
结论:PES在生物相容性和牙本质小管适应和穿透性方面显示了有希望的结果。
BACKGROUND: Endodontic sealers play a vital role in the obturation of root canal space. The aim of this study was to evaluate the utility of a recently developed polyurethane expandable sealer (PES), along with its cytotoxicity and dimensional changes.
METHODS: L929 fibroblasts and an cell viability assay (MTS assay) were used to determine the cytotoxicity of dental sealers (AH Plus [Dentsply Maillefer, Ballaigues, Switzerland], Sure-Seal Root [Sure Dent Corporation, Gyeonggi-do, South Korea], and the PES) at 24, 48, 72, and 96 hours. An advanced choroidal neovascularization model was used to assess the effect of these sealers on angiogenesis. Thirty-six extracted single-rooted human teeth were prepared and randomly divided into 3 groups (n = 12). Obturation was performed with gutta-percha and a sealer using lateral compaction as follows: group 1, AH Plus; group 2, Sure-Seal; and group 3, PES. The average depth of sealer penetration into dentinal tubules was measured with a scanning electron microscope. Data were analyzed using 1-way analysis of variance and post hoc Tukey tests (level of significance, P < .05).
RESULTS: The values of MTS, choroidal neovascularization, and the penetration depth of PES were significantly higher than in other experimental groups (P < .05). The lowest values were noted in specimens of AH Plus, whereas the highest were detected in the PES group.
CONCLUSIONS: PES showed promising results in terms of biocompatibility and dentinal tubule adaptation and penetration.