■近年来,许多研究表明,古代小麦产品可能对心脏代谢风险有有益的影响,但对它们对肠道微生物群(GM)的影响知之甚少。本研究的目的是评估与用现代小麦(CD)制成的面食替代饮食相比,用古代小麦(AD)制成的面食替代饮食是否会影响转基因成分及其代谢产物的产生。
■随机,对20名临床健康成人(9名女性;11名男性;平均年龄43.1±12.5岁)进行了包含两个干预阶段的双盲交叉试验.研究参与者被分配以随机顺序食用使用来自古代或现代对照小麦的半全面粉制成的面食8周。在干预措施之间实施了8周的清除期。在每个干预期开始和结束时从所有受试者收集粪便样品。GM成分,评估了短链脂肪酸(SCFA)和中链脂肪酸(MCFA)的产生。
■饮食干预并未在较高等级的GM组成中产生显着的多样性(门,类,秩序和家庭),但只在属级别。详细来说,明显的AD(adj。p<0.05)改变了丹毒杆菌的丰度。,拟杆菌属_果胶菌_群种。,CAG-873spp.,和Holdemanellaspp.CD显着影响了Akkermansia的丰度。,CAG-873spp.,Hungatellaspp.,落叶松科_UCG-008spp。,NK4A214_groupspp。,Frisingicocusspp.,Megasphaeraspp.,协同作用。,和Tyzzerellaspp.关于SCFA和MCFA的生产,AD导致粪便乙酸的显着增加(0.7%),异丁酸(+30.1%),2-甲基丁酸(+64.2%),和异戊酸(+22.5%)。另一方面,CD导致异丁酸水平增加(+71.4%),2-甲基丁酸(+116.2%),异戊(+99%),和戊酸(+21.4%),丁酸(~31.6%)和己酸(~66.4%)的减少。
■使用古代和现代小麦面食的短期替代饮食确定了属水平上转基因成分的显着变化,但值得注意的是,AD对抗炎SCFA产生了更大的有益影响。
UNASSIGNED: In recent years, many studies have suggested that ancient wheat products might have beneficial effects on cardiometabolic risk profile, but little is known about their effect on gut microbiota (GM). The aim of the present study was to evaluate whether a replacement diet with pasta made from ancient wheat (AD) could influence the GM composition and its metabolites\' production compared to a replacement diet with pasta made from modern wheat (CD).
UNASSIGNED: A randomized, double-blinded crossover trial with two intervention phases was conducted on 20 clinically healthy adults (9 females; 11 males; mean age 43.1 ± 12.5 years). Study participants were assigned to consume pasta made using semi-whole flour from organic wheat that was either from ancient or modern control wheat for 8 weeks in a random order. An 8-week washout period was implemented between the interventions. Stool samples were collected from all subjects at the beginning and at the end of each intervention period. GM composition, and short- (SCFAs) and medium- chain fatty acids (MCFAs) production was evaluated.
UNASSIGNED: Dietary interventions did not produce significant diversity in the GM composition at higher ranks (phylum, class, order and family), but only at genus level. In detail, the AD significantly (adj. p < 0.05) changed the abundance of Erysipelatoclostridium spp., Bacteroides_pectinophilus_group spp., CAG-873 spp., and Holdemanella spp. The CD significantly affected the abundance of Akkermansia spp., CAG-873 spp., Hungatella spp., Lachnospiraceae_UCG-008 spp., NK4A214_group spp., Frisingicoccus spp., Megasphaera spp., Synergistes spp., and Tyzzerella spp. Regarding the production of SCFAs and MCFAs, AD resulted in a significant increase of fecal acetic (+0.7%), isobutyric (+30.1%), 2-methylbutyric (+64.2%), and isovaleric (+22.5%) acids. On the other hand, CD resulted in increased levels of isobutyric (+71.4%), 2-methylbutyric (+116.2%), isovaleric (+99%), and valeric (+21.4%) acids, and a reduction of butyric (-31.6%) and hexanoic (-66.4%) acids.
UNASSIGNED: A short-term replacement diet with both ancient and modern wheat pasta determined significant changes in GM composition at the genus level but notably the AD resulted in a greater beneficial impact on anti-inflammatory SCFAs.