PDF(Pubmed)
Abstract：
Wheat allergy is a major type of food allergy with the potential for life-threatening anaphylactic reactions. Common wheat, Triticum aestivum (hexaploid, AABBDD genome), was developed using tetraploid wheat (AABB genome) and the ancient diploid wheat progenitor (DD genome)-Aegilops tauschii. The potential allergenicity of gluten from ancient diploid wheat is unknown. In this study, using a novel adjuvant-free gluten allergy mouse model, we tested the hypothesis that the glutenin extract from this ancient wheat progenitor will be intrinsically allergenic in this model. The ancient wheat was grown, and wheat berries were used to extract the glutenin for testing. A plant protein-free colony of Balb/c mice was established and used in this study. The intrinsic allergic sensitization potential of the glutenin was determined by measuring IgE response upon transdermal exposure without the use of an adjuvant. Clinical sensitization for eliciting systemic anaphylaxis (SA) was determined by quantifying the hypothermic shock response (HSR) and the mucosal mast cell response (MMCR) upon intraperitoneal injection. Glutenin extract elicited a robust and specific IgE response. Life-threatening SA associated and a significant MMCR were induced by the glutenin challenge. Furthermore, proteomic analysis of the spleen tissue revealed evidence of in vivo Th2 pathway activation. In addition, using a recently published fold-change analysis method, several immune markers positively and negatively associated with SA were identified. These results demonstrate for the first time that the glutenin from the ancient wheat progenitor is intrinsically allergenic, as it has the capacity to elicit clinical sensitization for anaphylaxis via activation of the Th2 pathway in vivo in mice.
摘要：
小麦过敏是一种主要的食物过敏，有可能危及生命的过敏反应。普通小麦,普通小麦（六倍体，AABBDD基因组），是使用四倍体小麦（AABB基因组）和古老的二倍体小麦祖细胞（DD基因组）-Aegilopstauschii开发的。来自古代二倍体小麦的面筋的潜在致敏性是未知的。在这项研究中,使用新型无佐剂麸质过敏小鼠模型，我们检验了这样一个假设，即在这个模型中，从这个古老的小麦祖先中提取的谷蛋白将具有内在的变应原性。古老的小麦种植，和小麦浆果被用来提取谷蛋白进行测试。建立了Balb/c小鼠的无植物蛋白菌落，并将其用于本研究。谷蛋白的内在变应性致敏潜力是通过在不使用佐剂的情况下测量经皮暴露时的IgE反应来确定的。通过定量腹膜内注射时的低温休克反应（HSR）和粘膜肥大细胞反应（MMCR）来确定引起全身过敏反应（SA）的临床敏化。谷蛋白提取物引起稳健和特异性的IgE反应。谷蛋白攻击诱导了威胁生命的SA相关和显著的MMCR。此外，脾组织的蛋白质组学分析揭示了体内Th2途径激活的证据。此外,使用最近发布的倍数变化分析方法，一些免疫标记与SA呈正相关和负相关。这些结果首次表明，来自古代小麦祖先的谷蛋白具有内在的过敏原，因为它有能力在小鼠体内通过激活Th2途径引起过敏反应的临床敏化。
