冷驯化过程受许多因素的调节,如环境温度,天长度,光强度,或荷尔蒙状态。在不同光质条件下生长的植物的实验表明,植物对寒冷的反应也是一个依赖于光质的过程。这里,在白色(100和20μmolm-2s-1)下,在短日条件下暴露于4°C的1个月大的拟南芥(Col-0)植物中研究了光质在冷响应中的作用,蓝色,或红色(20μmolm-2s-1)光照条件。CBF1的上调表达,抑制光合作用,膜损伤的增加表明蓝光增强了低温的作用。有趣的是,与白光冷处理的植物相比,蓝光和红光下冷处理的植物仅显示出有限的耐冻性。接下来,在来自不同和对比纬度的拟南芥种质中评估了冷响应中光质信号的特异性。除了一个拟南芥,蓝光增加了寒冷对光合参数和电解质渗漏的影响。对于缺乏功能性CRY2蛋白的Ws-0没有发现这种作用,表明它在寒冷反应中的作用。蛋白质组学数据证实了低温下红光和蓝光处理的植物之间的显着差异,并表明冷反应具有高度的登录性。总的来说,蓝光主要增加冷胁迫相关蛋白,红光诱导叶绿体相关蛋白的高表达,这与红光冷处理植物中较高的光合参数相关。总之,我们的数据表明,在冷处理期间,光调节两种不同的机制-红光驱动的细胞功能维持程序和蓝光激活的特定冷反应.通过在白光下冷处理的植物的显着更高的耐冻性证明了这些机制相互互补的重要性。
The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in 1-month-old Arabidopsis thaliana (Col-0) plants exposed for 1 week to 4°C at short-day conditions under white (100 and 20 μmol m-2s-1), blue, or red (20 μmol m-2s-1) light conditions. An upregulated expression of CBF1, inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis
accession, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light-treated plants at low temperatures and showed that the cold response is highly
accession-specific. In general, blue light increased mainly the cold-stress-related proteins and red light-induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light-driven cell function maintaining program and blue light-activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.