萎缩芽孢杆菌HAB-5是一种植物生长促进根际细菌(PGPR),具有多种生物技术特征,例如促进植物生长,在根际定居,从事生物防治活动。在这项研究中,我们使用PacificBiosciences(PacBio;UnitedStates)的单分子实时(SMRT)测序平台对B.atrophaeusHAB-5进行了全基因组测序,其具有环状染色体,总长度为4,083,597bp,G+C含量为44.21%。与其他菌株的比较基因组分析。解淀粉芽孢杆菌DSM7、萎缩芽孢杆菌SRCM101359、变形杆菌FZB42、变形杆菌HAB-2和枯草芽孢杆菌168表明,这些菌株共有2,465个CDS,而599个CDS是萎缩芽孢杆菌HAB-5株独有的。萎缩芽孢杆菌HAB-5基因组中的许多基因簇与抗微生物脂肽和多肽的产生相关。这些基因簇包含编码三个NRP的不同酶,两个Transat-Pks,一种萜烯,一种羊毛硫肽,一个T3PKS,一个Ripp,和一个硫肽。除了可能产生IAA的基因(trpA,trpB,trpC,trpD,trpE,trps,ywkB,MiaA,andnadE),有可能产生挥发性化学物质的基因(acoA,acoB,acoR,acuB,和acuC)。此外,HAB-5含有与铁运输相关的基因(fbpA,fetB,FeuC,feub,FeuA,和fecD),硫代谢(cysC,sat,cysK,cysS,andsulP),磷溶解(ispH,pstA,pstC,pstS,pstB,gltP,和phoH),和固氮(类似于nif3,gltP,gltX,glnR,glnA,nadR,nirB,nird,美国国家航空航天局,narl,narh,narJ,和纳克)。总之,这项研究提供了一个全面的基因组分析B.abrophaeusHAB-5,精确定位的基因和基因组区域相关的抗菌特性的菌株。这些发现提高了我们对枯萎病芽孢杆菌抗菌特性的遗传基础的认识,并暗示HAB-5可以采用各种商业生物农药和生物肥料作为替代策略来增加农业产量和管理各种植物病害。
Bacillus atrophaeus HAB-5 is a plant growth-promoting rhizobacterium (PGPR) that exhibits several biotechnological traits, such as enhancing plant growth, colonizing the rhizosphere, and engaging in biocontrol activities. In this study, we conducted whole-genome sequencing of B. atrophaeus HAB-5 using the single-molecule real-time (SMRT) sequencing platform by Pacific Biosciences (PacBio; United States), which has a circular chromosome with a total length of 4,083,597 bp and a G + C content of 44.21%. The comparative genomic analysis of B. atrophaeus HAB-5 with other strains, Bacillus amyloliquefaciens DSM7, B. atrophaeus SRCM101359, Bacillus velezensis FZB42, B. velezensis HAB-2, and Bacillus subtilis 168, revealed that these strains share 2,465 CDSs, while 599 CDSs are exclusive to the B. atrophaeus HAB-5 strain. Many gene clusters in the B. atrophaeus HAB-5 genome are associated with the production of antimicrobial lipopeptides and polypeptides. These gene clusters comprise distinct enzymes that encode three NRPs, two Transat-Pks, one terpene, one lanthipeptide, one T3PKS, one Ripp, and one thiopeptide. In addition to the likely IAA-producing genes (trpA, trpB, trpC, trpD, trpE, trpS, ywkB, miaA, and nadE), there are probable genes that produce volatile chemicals (acoA, acoB, acoR, acuB, and acuC). Moreover, HAB-5 contained genes linked to iron transportation (fbpA, fetB, feuC, feuB, feuA, and fecD), sulfur metabolism (cysC, sat, cysK, cysS, and sulP), phosphorus solubilization (ispH, pstA, pstC, pstS, pstB, gltP, and phoH), and nitrogen fixation (nif3-like, gltP, gltX, glnR, glnA, nadR, nirB, nirD, nasD, narl, narH, narJ, and nark). In conclusion, this study provides a comprehensive genomic analysis of B. atrophaeus HAB-5, pinpointing the genes and genomic regions linked to the antimicrobial properties of the strain. These findings advance our knowledge of the genetic basis of the antimicrobial properties of B. atrophaeus and imply that HAB-5 may employ a variety of commercial biopesticides and biofertilizers as a substitute strategy to increase agricultural output and manage a variety of plant diseases.