研究的目的是分析葡萄品种自然变异性的选定方面。由于葡萄酒生产的悠久传统,葡萄是世界上最受欢迎和最受欢迎的作物之一,但葡萄提取物对人体也有广泛的药效作用。确定不同的品种对保护遗传资源很重要,但也用于商业和种植目的。保守的DNA衍生多态性谱的变异性,以及微生物特征,在这项研究中进行了分析。研究中使用了六种不同的葡萄品种:赤霞珠,Chardonney,Welschriesling,WeisserRiesling,Gewurztramines和GrunerVeltliner。通过CDDP标记分析WRKY基因的遗传多态性。通过研究中使用的所有引物组合产生多态扩增子谱。GrunerVeltliner和Welschriesling最相似,相似值为0.778。进一步分析了葡萄的微生物学质量以及对革兰氏阳性和革兰氏阴性细菌和酵母的抗菌活性。评估了用于微生物质量的平板稀释方法和用于抗菌活性的圆盘扩散方法。赤霞珠的细菌总数在3.12至3.62logcfu/g之间。Gewurztramine对沙门氏菌的抗菌活性最好,小肠结肠炎耶尔森氏菌,铜绿假单胞菌,金黄色葡萄球菌,单核细胞增生李斯特菌,白色念珠菌,克鲁斯念珠菌,和热带念珠菌.在WeisserRiesling品种中发现了对粪肠球菌的最佳抗菌活性。
The aim of the study was to analyse selected aspects of the natural variability of selected varieties of Vitis vinifera. Grapevine is one of the most popular and desirable crops in the world due to the great tradition of wine production, but grape extracts also have a wide range of pharmaceutical effects on the human body. It is important to identify different varieties for the conservation of genetic resources, but also for commercial and cultivation purposes. The variability of conserved DNA-derived polymorphism profiles, as well as microbial characteristics, were analysed in this study. Six different varieties of Vitis vinifera L. were used in the study: Cabernet Savignon, Chardonney, Welschriesling, Weisser Riesling, Gewurztramines and Gruner Veltliner. Genetic polymorphism was analysed by CDDP markers for WRKY genes. Polymorphic amplicon profiles were generated by all primer combinations used in the study. Gruner Veltliner and Welschriesling were the most similar, with a similarity value at 0.778. Microbiological quality of grape and antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts were analysed further. The plate diluting method for microbial quality and the disc diffusion method for antimicrobial activity were evaluated. The number of total count of bacteria ranged between 3.12 in Cabernet Sauvignon to 3.62 log cfu/g in Grűner Veltliner. The best antimicrobial activity showed Gewurztramines against Salmonella enterica, Yersinia enterocolitica, Pseudomonas aeroginosa, Staphylococcus aureus, Listeria monocytogenes, Candida albicans, Candida krusei, and Candida tropicalis. The best antimicrobial activity was found against Enterococcus faecalis in variety Weisser Riesling.