由于潜在的不利健康影响,人类通过饮用水接触微塑料(MP)最近引起了严重关注。尽管有报道称瓶装水中出现国会议员,由于常规MPs检测方法的限制,对整个范围从1µm到5mm的MPs的丰度知之甚少。一些使用显微拉曼光谱的研究可以获得尺寸<10μm的MPs,然而,所有MPs的定量是非常耗时的,只有一小部分(<10%)的MPs将被分析.本研究使用荧光显微镜和流式细胞术对9种瓶装水的MPs进行了定量,其中MPs的大小≥50µm,<50µm。分别。发现瓶装水样品中大小≥50µm的MPs的平均丰度为8-50个颗粒L-1,而大小<50µm的MPs为1570-17,817个颗粒L-1,其中矿泉水样品中的MPs丰度明显高于蒸馏水和泉水样品。发现MPs的模态大小和形状为1μm和碎片,分别。此外,对当地获得的三个自来水样品进行了分析,并与瓶装水样品进行了比较,在自来水样本中发现的议员较少。此外,研究了瓶盖和瓶盖对MP的污染以及添加矿物盐的干扰,在所有这些过程与对照样品之间没有发现显着差异,这表明MP的主要污染来自其他制造过程。当包括小MP数据时,MP的估计每日摄入量(EDI)大幅增加,这表明以前关于国会议员从饮用水中暴露的报道可能被低估了,因为只考虑了大议员。
Human exposure to microplastics (MPs) through drinking water has drawn serious concern recently because of the potential adverse health effects. Although there are reports on the occurrence of MPs in bottled water, little is known about the abundance of a whole spectrum of MPs with sizes ranging from 1 µm to 5 mm due to the restrictions of conventional MPs detection methods. Some studies using micro-Raman spectroscopy can achieve MPs with a size of <10 µm, however, quantitation of all MPs was extremely time consuming and only a small portion (<10%) of MPs would be analyzed. The present study quantified MPs from nine brands of bottled water using fluorescence microscopy and flow cytometry for MPs with a size of ≥50 µm and a size of <50 µm, respectively. The average abundance of MPs with a size of ≥50 µm in bottled water samples was found ranging from 8-50 particles L-1, while MPs with a size of <50 µm were found to be 1570-17,817 particles L-1, where the MPs abundance from mineral water samples were significantly more than distilled and spring water samples. The modal size and shape of MPs were found at 1 µm and fragments, respectively. Besides, three tap water samples obtained locally were analyzed and compared with the bottled water samples, where less MPs were found in tap water samples. In addition, contamination of MPs from bottle and cap and interference by addition of mineral salts were studied, where no significant difference from all these processes to the control sample was found, suggesting the major contamination of MPs was from other manufacturing processes. Estimated daily intake (EDI) of MPs increased substantially when data of small MPs are included, suggesting that previously reports on exposure of MPs from drinking water might be underestimated, as only large MPs were considered.