黄萎病,由黄萎病菌引起,是一种影响茄子的土壤传播疾病。野生茄子,被认为是抗黄萎病的优良抗病资源,在嫁接和抗病育种中起着举足轻重的作用。然而,野生茄子的基本抗性机制仍然知之甚少。本研究比较了两个野生茄子品种,LC-2(高抗性)和LC-7(敏感)在表型,转录组,和代谢组学水平来确定其对黄萎病抗性的分子基础。这两个品种在花瓣颜色上表现出实质性的表型差异,叶刺,和果实性状。接种大丽花弧菌后,LC-2显示出显著较高的多酚氧化酶活性,超氧化物歧化酶,过氧化物酶,苯丙氨酸氨裂解酶,β-1,3葡聚糖酶,和几丁质酶比LC-7。RNA测序显示4,017个差异表达基因(DEG),其中很大一部分涉及与疾病抗性和生长相关的过程。这些过程包括防御反应,细胞壁生物发生,发展过程,和亚精胺的生物合成,肉桂酸,还有角质.基因共表达分析确定了13个转录因子作为与植物防御反应相关的模块中的枢纽基因。一些基因在LC-2和LC-7之间表现出不同的表达模式,表明它们在响应感染中的关键作用。Further,代谢组分析确定了LC-2和LC-7之间的549种差异积累的代谢物(DAM),主要由类黄酮等化合物组成,酚酸,脂质,和其他代谢物。整合的转录组和代谢组分析揭示了与植物防御反应相关的模块中35个基因-代谢物对的关联,强调植物防御反应背后的相互联系的过程。这些发现表征了LC-2抗黄萎病的分子基础,因此对未来抗枯萎病茄子品种的选育具有潜在价值。
Verticillium wilt, caused by Verticillium dahliae, is a soil-borne disease affecting eggplant. Wild eggplant, recognized as an excellent disease-resistant resource against verticillium wilt, plays a pivotal role in grafting and breeding for disease resistance. However, the underlying resistance mechanisms of wild eggplant remain poorly understood. This study compared two wild eggplant varieties, LC-2 (high resistance) and LC-7 (sensitive) at the phenotypic, transcriptomic, and metabolomic levels to determine the molecular basis of their resistance to verticillium wilt. These two varieties exhibit substantial phenotypic differences in petal color, leaf spines, and fruit traits. Following inoculation with V. dahliae, LC-2 demonstrated significantly higher activities of polyphenol oxidase, superoxide dismutase, peroxidase, phenylalanine ammonia lyase, β-1,3 glucanase, and chitinase than did LC-7. RNA sequencing revealed 4,017 differentially expressed genes (DEGs), with a significant portion implicated in processes associated with disease resistance and growth. These processes encompassed defense responses, cell wall biogenesis, developmental processes, and biosynthesis of spermidine, cinnamic acid, and cutin. A gene co-expression analysis identified 13 transcription factors as hub genes in modules related to plant defense response. Some genes exhibited distinct expression patterns between LC-2 and LC-7, suggesting their crucial roles in responding to infection. Further, metabolome analysis identified 549 differentially accumulated metabolites (DAMs) between LC-2 and LC-7, primarily consisting of compounds such as flavonoids, phenolic acids, lipids, and other metabolites. Integrated transcriptome and metabolome analyses revealed the association of 35 gene-metabolite pairs in modules related to the plant defense response, highlighting the interconnected processes underlying the plant defense response. These findings characterize the molecular basis of LC-2 resistance to verticillium wilt and thus have potential value for future breeding of wilt-resistant eggplant varieties.