PDF(Pubmed)
Abstract：
Cotton is one of the world\'s most important economic crops. Verticillium wilt is a devastating cotton disease caused by Verticillium dahliae, significantly impacting cotton yield and quality. E3 ubiquitin ligases are essential components of the ubiquitin-mediated 26S proteasome system, responsible for recognizing ubiquitinated target proteins and promoting their degradation, which play a crucial regulatory role in plant immune responses. In this study, on the basis of the confirmation of differential expression of GhDIRP1, a RING-type E3 ubiquitin ligase encoding gene, in two cotton varieties resistant (Zhongzhimian 2) or susceptible (Jimian 11) to V. dahliae, we demonstrated that GhDIRP1 is a negative regulator of V. dahliae resistance because silencing GhDIRP1 in cotton and heterogeneously overexpressing the gene in Arabidopsis enhanced and compromised resistance to V. dahliae, respectively. The GhDIRP1-mediated immune response seemed to be realized through multiple physiological pathways, including hormone signaling, reactive oxygen species, and lignin biosynthesis. Based on the sequences of GhDIRP1 isolated from Zhongzhimian 2 and Jimian 11, we found that GhDIRP1 had identical coding but different promoter sequences in the two varieties, with the promoter of Zhongzhimian 2 being more active than that of Jimian 11 because the former drove a stronger expression of GUS and LUC reporter genes. The results link the ubiquitination pathway to multiple physiological pathways acting in the cotton immune response and provide a candidate gene for breeding cotton varieties resistant to V. dahliae.
摘要：
棉花是世界上最重要的经济作物之一。黄萎病是由大丽花黄萎病引起的一种毁灭性的棉花病害，影响棉花产量和品质。E3泛素连接酶是泛素介导的26S蛋白酶体系统的重要组成部分，负责识别泛素化的靶蛋白并促进其降解，在植物免疫反应中起着至关重要的调节作用。在这项研究中,在GhDIRP1的差异表达的基础上，一个RING型E3泛素连接酶编码基因，在两个对大丽花有抗性的棉花品种(中志棉2号)或易感的棉花品种(吉棉11号)中，我们证明了GhDIRP1是V.dahliae抗性的负调节因子，因为在棉花中沉默GhDIRP1并在拟南芥中异质过表达该基因会增强并损害对V.dahliae的抗性，分别。GhDIRP1介导的免疫应答似乎是通过多种生理途径实现的。包括激素信号,活性氧，和木质素生物合成。根据从中志棉2号和集棉11号分离的GhDIRP1序列，我们发现GhDIRP1在两个品种中具有相同的编码但不同的启动子序列，中直勉2的启动子比Jimian11的启动子更活跃，因为前者驱动了GUS和LUC报告基因的更强表达。该结果将泛素化途径与作用于棉花免疫应答的多种生理途径联系起来，并为培育抗大丽花的棉花品种提供了候选基因。
