背景:由于其毒性作用和潜在的药物应用,维拉生物碱引起了人们的关注,特别是在癌症和心脏病学。在Veratum属的物种中发现了200多种生物碱。生物碱的组成和浓度在植物中可能会有很大差异,这取决于物种等因素,植物部分,location,季节,天气,或营养素。
目的:本研究旨在通过一种分析方法来分析和定量Veratum物种不同植物部位的Veratum生物碱。目的是为未来有关Veratum生物碱的药理和毒理学方面的研究提供必要的生物碱浓度数据。
方法:本研究的重点是五种Veratrum生物碱(cevadine,Jervine,protveratrineA,veratramine,和veratridine)在三种Veratrum物种中(Veratrum专辑L.,杜兰德,和VeratrumnigrumL.)从四个德国植物园(德累斯顿,莱比锡,马尔堡,和Schellerhau)。采用液-液萃取法和灵敏的高效液相色谱-串联质谱(HPLC-MS/MS)方法在多反应监测(MRM)模式下进行生物碱测定。
结果:定量显示植物部位和Veratum物种之间的生物碱浓度在μg/g至mg/g范围内变化。原草A含量最高,而维拉他明浓度普遍较低。尤其是水果,Veratrum的根和砧木生物碱浓度很高。
结论:所开发的HPLC-MS/MS方法成功地测定了植物样品中Veratum生物碱的浓度。该研究为Veratum生物碱在不同物种和植物部位的分布提供了宝贵的数据,对于了解其潜在的医学和毒理学意义至关重要。
BACKGROUND: Veratrum alkaloids have gained attention due to their toxic effects and potential pharmaceutical applications, particularly in cancer and cardiology. Over 200 alkaloids are found in species of the Veratrum genus. The alkaloid composition and concentrations can greatly vary in plants depending on factors like species, plant part, location, season, weather, or nutrients.
OBJECTIVE: This study aims an analytical approach to analyze and quantify Veratrum alkaloids in different plant parts of Veratrum species. The purpose is to contribute essential alkaloid concentration data for future research on the pharmacological and toxicological aspects of Veratrum alkaloids.
METHODS: This study focuses on five Veratrum alkaloids (cevadine, jervine, protoveratrine A, veratramine, and veratridine) in three Veratrum species (Veratrum album L., Veratrum californicum Durand, and Veratrum nigrum L.) collected from four German botanical gardens (Dresden, Leipzig, Marburg, and Schellerhau). A liquid-liquid extraction method and a sensitive high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) method operating in multiple reaction monitoring (MRM) mode were applied for the alkaloid determination.
RESULTS: Quantification revealed varying alkaloid concentrations among plant parts and Veratrum species in the μg/g to mg/g range. Protoveratrine A exhibited the highest content, while veratramine concentrations were generally lower. Especially in fruit, roots and rootstock of Veratrum album L. alkaloid concentrations were significant high.
CONCLUSIONS: The developed HPLC-MS/MS method successfully determined Veratrum alkaloid concentrations in plant samples. The study contributes valuable data on Veratrum alkaloid distribution in different species and plant parts, crucial for understanding their potential medicinal and toxicological significance.