生态化学计量学和化学计量稳态的知识可以有助于探索生态恢复中化学元素的平衡。然而,目前还不清楚碳(C),氮(N),磷(P),植物-土壤-微生物连续系统中的化学计量特征响应退化的高山草地的自发次生演替。因此,我们调查了青藏高原上zokor(Myospalaxfontanierii)干扰的草地的自发次生演替恢复,中国,通过用空间代替时间的策略。基于植物的丰富度,生物量,和覆盖范围,植物重要性值用于评估zokor制成的土墩的恢复程度(ZMMs,由zokors建造的大而裸的补丁区域)。多种统计方法,包括化学计量稳态模型,网络,和冗余分析,进行破译化学计量模式。结果表明,植物C,C:N,C:P随着ZMMs的回收而增加,与植物N和P的减少相反,土壤C,N,C:N,C:P,N:P随恢复程度增加,在豆科植物的重新植被下,通过增加有机氮,土壤变得相对更富含氮。同时,土壤微生物生物量C,N,P随着ZMMs的回收而增加,但微生物生物量C:N:P比例受到高度限制。土壤可利用的无机氮在驱动植物和微生物养分和化学计量中发挥着重要作用。我们的结果表明,C的不同反应,N,植物-土壤-微生物中的P含量导致C:N:P化学计量比的变化。然而,植物和土壤微生物表现出强烈的化学计量稳态。总的来说,我们的研究从化学计量角度为青藏高原退化高寒草地演替恢复的生物地球化学响应提供了新的见解。
The knowledge of ecological stoichiometry and stoichiometric homeostasis could contribute to exploring the balance of chemical elements in ecological recovery. However, it is largely unknown how the carbon (C), nitrogen (N), phosphorus (P), and stoichiometric characteristics in the plant-soil-microbe continuum system respond to the spontaneous secondary succession of degraded alpine grasslands. Therefore, we investigated the spontaneous secondary successional recovery of grasslands disturbed by zokor (Myospalax fontanierii) on the Qinghai-Tibetan Plateau, China, via a strategy of substituting space for time. Based on plant richness, biomass, and coverage, plant importance value was employed to assess the recovery degree of zokor-made mounds (ZMMs, large and bare patch areas constructed by zokors). Multiple statistical methods, including stoichiometric homeostatic model, network, and redundancy analysis, were conducted to decipher the stoichiometric patterns. The results indicated that plant C, C:N, and C:P increased with the recovery of ZMMs, contrary to the decrease of plant N and P. In addition, soil C, N, C:N, C:P, and N:P increased with the recovery degree, and the soil became relatively more N rich by increasing organic N under the revegetation of legumes. Meanwhile, soil microbial biomass C, N, and P increased with the recovery of ZMMs, but microbial biomass C:N:P ratios were highly constrained. Soil accessible inorganic nitrogen played an important role in driving plant and microbial nutrient and stoichiometry. Our results demonstrated that the different responses of C, N, and P contents in plant-soil-microbe lead to shifts in C:N:P stoichiometric ratio. Nevertheless, plants and soil microbes exhibited strong stoichiometric homeostasis. Collectively, our study provides new insight into biogeochemical responses to the successional recovery of degraded alpine grassland on the Qinghai-Tibetan Plateau from a stoichiometric perspective.