在这项工作中,我们重点分析了唾液中VEGF的含量及其与乳腺癌免疫调节和血管生成相关的促炎细胞因子和氨基酸的关系.该研究包括230名乳腺癌患者,92例良性乳腺疾病患者,和59个健康对照。治疗前,唾液样本来自所有参与者,通过酶联免疫吸附法测定唾液中VEGF和细胞因子的含量,以及高效液相色谱法测定氨基酸的含量。发现VEGF与促炎细胞因子IL-1β水平呈正相关(r=0.6367),IL-6(r=0.3813),IL-8(r=0.4370),和IL-18(r=0.4184)。MCP-1(r=0.2663)和TNF-α(r=0.2817)的相关性较弱。第一次,我们证明了不同分子生物学亚型乳腺癌唾液中VEGF和相关细胞因子的浓度变化,分化,扩散,和淋巴结转移。VEGF的表达与天冬氨酸含量之间建立了相关性(r=-0.3050)。瓜氨酸(r=-0.2914),和唾液中的色氨酸(r=0.3382)。已经提出天冬氨酸和瓜氨酸通过合成信号分子NO影响VEGF的表达,然后色氨酸确保免疫系统对肿瘤细胞的耐受性。
In this work, we focused on the analysis of
VEGF content in saliva and its relationship with pro-inflammatory cytokines and amino acids involved in immunomodulation and angiogenesis in breast cancer. The study included 230 breast cancer patients, 92 patients with benign breast disease, and 59 healthy controls. Before treatment, saliva samples were obtained from all participants, and the content of
VEGF and cytokines in saliva was determined by an enzyme-linked immunosorbent assay, as well as the content of amino acids by high-performance liquid chromatography. It was found that
VEGF was positively correlated with the level of pro-inflammatory cytokines IL-1β (r = 0.6367), IL-6 (r = 0.3813), IL-8 (r = 0.4370), and IL-18 (r = 0.4184). Weak correlations were shown for MCP-1 (r = 0.2663) and TNF-α (r = 0.2817). For the first time, we demonstrated changes in the concentration of
VEGF and related cytokines in saliva in different molecular biological subtypes of breast cancer depending on the stage of the disease, differentiation, proliferation, and metastasis to the lymph nodes. A correlation was established between the expression of
VEGF and the content of aspartic acid (r = -0.3050), citrulline (r = -0.2914), and tryptophan (r = 0.3382) in saliva. It has been suggested that aspartic acid and citrulline influence the expression of VEGF via the synthesis of the signaling molecule NO, and then tryptophan ensures tolerance of the immune system to tumor cells.