■在一定压力下进行腹腔镜手术有可能导致腹腔内粘连。然而,这种疾病的发病机制尚不清楚.肥大细胞脱颗粒导致的肥大细胞介质的释放被认为是原因。
■将30只雄性Sprague-Dawley大鼠分为五组(每组n=6):一个对照组和四个干预组,分别使用5、8、10和12mmHg的二氧化碳进行60分钟的吹气。腹腔镜检查后七天,我们安乐死并评估了组胺的水平,胰蛋白酶,和腹腔液的糜蛋白酶,腹膜组织ECM的厚度,和腹腔粘连评分系统。
■在10-和12mmHg干预下,腹膜液中的组胺和类胰蛋白酶水平明显高于对照组(组胺:0.50±0.35vs.0.41±0.41vs.0.04±0.02ng/mL,分别;和类胰蛋白酶:0.69±0.11vs.0.65±0.05vs.分别为0.48±0.02ng/ml)。与对照组相比,干预组的ECM在10和12mmHg时明显增厚(71.3[66.7-85.2]vs.48.4[34.5-50.3]vs.10.25[8.7-12.1]μm,分别)。此外,与对照组相比,干预组在10和12mmHg时的腹内评分也显着较高(4[0-4]与4.5[4-5],vs.分别为0)。
■腹腔镜手术增加了腹膜液中肥大细胞介质的释放,大鼠ECM厚度和腹腔粘连评分,暗示它可能会增加人体腹内粘连的可能性。
UNASSIGNED: Laparoscopic procedures under certain pressure have the potential to cause intra-abdominal adhesions. However, the pathomechanism of this disorder is unknown. Release of mast cell mediators due to mast cell degranulation is thought to be the cause.
UNASSIGNED: Thirty male Sprague-Dawley rats were grouped into five groups (n = 6 per group): one control group and four intervention groups to which 60 min insufflation was performed using carbon dioxide at 5, 8, 10 and 12 mmHg. Seven days after laparoscopy, we euthanized and evaluated the levels of histamine, tryptase, and chymase of peritoneal fluid, the thickness of ECM of peritoneal tissue, and intraabdominal adhesion scoring system.
UNASSIGNED: Histamine and tryptase levels in peritoneal fluid were significantly higher at the 10- and 12 mm Hg intervention compared to control (histamine: 0.50 ± 0.35 vs. 0.41 ± 0.41 vs. 0.04 ± 0.02 ng/mL, respectively; and tryptase: 0.69 ± 0.11 vs. 0.65 ± 0.05 vs. 0.48 ± 0.02 ng/ml respectively). The ECM was significantly thicker in the intervention groups at 10- and 12-mm Hg compared to control (71.3 [66.7-85.2] vs. 48.4 [34.5-50.3] vs. 10.25 [8.7-12.1] μm, respectively). Moreover, the intra-abdominal scoring was also significantly higher in the intervention groups at 10- and 12 mm Hg compared to control (4 [0-4] vs. 4.5 [4-5], vs. 0, respectively).
UNASSIGNED: Laparoscopic procedures increase the release of mast cell mediators in peritoneal fluid, the thickness of ECM and intraabdominal adhesion scoring in rats, implying that it might increase the possibility of intrabdominal adhesion in humans.