■本研究旨在探讨多重PCR检测在疑似尿道炎及相关症状的男性泌尿生殖系统样本中阴道加德纳菌的检出率。
■在2021年2月至2021年10月期间,共有144名男性患者出现泌尿系症状或无保护性行为后的担忧,包括在研究中。共有128份(88.9%)首次空尿样,15例(10.4%)尿道拭子,并获得一份(0.7%)精液样本。NeoPlexSTI-14检测多重PCR试剂盒(GeneMatrixInc.,韩国)用于调查以下任何病原体:白色念珠菌,沙眼衣原体,G.阴道,生殖支原体,人型支原体,淋病奈瑟菌,阴道毛滴虫,细小脲原体,解脲脲原体,单纯疱疹病毒1型(HSV-1),单纯疱疹病毒2型(HSV-2),梅毒螺旋体,无乳链球菌,还有杜克雷嗜血杆菌.对阴道G.阳性结果的患者进行回顾性分析。
■患者的中位年龄为37岁(范围:21至71岁)。G.vaginalis是最常见的微生物(n=23;15.9%)。按频率顺序发现的其他微生物是解脲杆菌(n=19;13.2%),U.parvum(n=15;10.4%),沙眼衣原体(n=11;7.6%),生殖M.(n=8;5.6%),HSV-2(n=7;4.9%),淋病奈瑟菌(n=6;4.2),HSV-1(n=2;1.4%),人马(n=1,0.7%),和白色念珠菌(n=1,0.7%)。15例患者(65%)对一种或两种微生物剂和阴道毛虫呈阳性,而在八名患者(35%)中,阴道球菌是唯一分离的药剂。这8名患者中有6名,其余15名中有14名是有症状的。
■随着多重PCR测试的引入,包括阴道G.我们可以预期男性泌尿生殖系统样本中这些细菌的检出率更高,这可能是无法解释的泌尿/尿道症状的原因。
UNASSIGNED: This study aimed to investigate the detection rate of Gardnerella vaginalis by multiplex PCR test in the genitourinary samples of male patients with suspected
urethritis and related symptoms.
UNASSIGNED: A total of 144 male patients who presented to our department between February 2021 and October 2021, either with urinary symptoms or concerns following unprotected sex, were included in the study.A total of 128 (88.9%) first-void urine samples, 15 (10.4%) urethral swabs, and one (0.7%) semen sample were obtained. NeoPlex STI-14 Detection Multiplex PCR Kit (GeneMatrix Inc. Seongnam, South Korea) was used to investigate any of the following pathogens: Candida albicans, Chlamydia trachomatis, G. vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum,herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), Treponema pallidum , Streptococcus agalactiae, and Haemophilus ducreyi. The patients with positive results for G. vaginalis were retrospectively analyzed.
UNASSIGNED: The patients\' median age was 37 (range: 21 to 71 years old). G. vaginalis was the most frequently detected microorganism (n=23; 15.9%). Other microorganisms found in order of frequency were U. urealyticum (n=19; 13.2%), U. parvum (n=15; 10.4%), C. trachomatis (n=11; 7.6%), M. genitalium (n=8; 5.6%), HSV-2 (n= 7; 4.9%), N. gonorrhoeae (n=6; 4.2), HSV-1 (n=2; 1.4%), M. hominis (n=1, 0.7%), and C. albicans (n=1, 0.7%). Fifteen patients (65%) were positive for one or two microbial agents together with G. vaginalis, while in eight patients (35%), G. vaginalis was the only isolated agent. Six of these eight patients and 14 of the remaining 15 were symptomatic.
UNASSIGNED: With the introduction of multiplex PCR tests, including those for G. vaginalis, we can expect a higher detection rate of these species of bacteria in male genitourinary samples, which could be the cause of unexplained urinary/urethral symptoms.