目的:非甾体抗炎药(NSAIDs),最广泛使用的药物,引起各种不良影响,包括胃肠道损伤,如溃疡和出血。NSAID诱导的小肠损伤(NSI)的动物模型已广泛用于开发预防和治疗剂。然而,NSI诱导后,观察到一些与进食时间有关的实验变化。本研究旨在探讨喂养时间对NSI小鼠模型的影响。
方法:将小鼠分为8组:sham,和模型组(喂食时间为2小时,6h,10h,14h,18h,和22小时;每组n=10)。在注射吲哚美辛(15mg/kg,皮下),除了正常组。在特定时间点停止食物供应(2小时,6h,10h,14h,18h,和22小时);然而,在整个实验过程中,正常组和假组均连续喂食。测量小肠的长度,诱导后24小时进行组织学分析。
结果:诱导后14小时,NSI,表现为小肠缩短,保持一致,长度减少约10-20%。然而,喂食超过14小时显著加剧了NSI,在解剖学和组织学上。
结论:吲哚美辛注射后14h小肠溃疡的变化可能与食物对NSI的影响密切相关。
OBJECTIVE: Non-steroidal anti-inflammatory drugs (NSAIDs), the most widely used pharmaceuticals, induce various adverse effects, including gastrointestinal injuries, such as ulcers and bleeding. Animal models of NSAID-induced small intestinal injury (NSI) have been extensively employed for the development of preventive and therapeutic agents. However, some experimental variations related to feeding times have been observed following NSI induction. This study aimed to investigate the impact of feeding time on an NSI mouse model.
METHODS: The mice were divided into eight groups: normal, sham, and model groups (with feeding times of 2 h, 6 h, 10 h, 14 h, 18 h, and 22 h; n=10 in each group). The mice were fasted for 18 h before the injection of indomethacin (15 mg/kg, subcutaneously), except for the normal group. Food supply was halted at specific time points (2 h, 6 h, 10 h, 14 h, 18 h, and 22 h); however, the normal and sham groups were continuously fed throughout the experiment. The length of the small intestine was measured, and histological analysis was performed 24 h after induction.
RESULTS: Up to 14 h after induction, NSI, indicated by small intestine shortening, remained consistent, with a reduction in length of approximately 10-20%. However, feeding for more than 14 h significantly exacerbated NSI, both anatomically and histologically.
CONCLUSIONS: The ulcerative changes observed in the small intestine 14 h after indomethacin injection may be closely associated with the influence of food on NSI.