UBiA基因编码一大类异戊烯基转移酶,它们参与叶绿素和维生素E等次生代谢产物的合成。它们在整个植物的生长发育中起着重要的作用。目前对UBiA基因的研究还不够全面,特别是向日葵UBiA基因。在这项研究中,通过结构域分析鉴定了10个HaUBiA,这些HaUBiA具有5个主要的保守结构域,并且不均匀地分布在6个染色体上。通过构建系统发育树,在12个具有不同进化水平的物种中发现了119个UBiA基因,并分为五个主要类群,其中包含七个保守基序和八个UBiA亚超家族域。组织表达分析表明HaUBiAs在根中高表达,叶子,和种子。通过使用启动子分析,UBiA基因的顺式元件主要表现在激素信号和应激反应中。qRT-PCR结果表明HaUBiA1和HaUBiA5对非生物胁迫反应强烈。在ABA和MeJA处理下,HaUBiA1显著上调,而HaUBiA5显著下降。在寒冷的压力下,UBiA1的表达在根和茎中显著上调,而UBiA5表达仅在叶片中增加。在厌氧诱导下,UBiA1和UBiA5在根中都上调,茎和叶。总之,本研究对向日葵中的UBiA家族进行了系统的分类,并鉴定了两个非生物胁迫候选基因。它扩展了对UBiA家族的认识,并为未来向日葵的非生物胁迫研究提供了理论依据。
The
UBiA genes encode a large class of isopentenyltransferases, which are involved in the synthesis of secondary metabolites such as chlorophyll and vitamin E. They performed important functions in the whole plant\'s growth and development. Current studies on
UBiA genes were not comprehensive enough, especially for sunflower
UBiA genes. In this study, 10 HaUBiAs were identified by domain analysis these HaUBiAs had five major conserved domains and were unevenly distributed on six chromosomes. By constructing phylogenetic trees, 119
UBiA genes were found in 12 species with different evolutionary levels and divided into five major groups, which contained seven conserved motifs and eight
UBiA subsuper family domains. Tissue expression analysis showed that HaUBiAs were highly expressed in the roots, leaves, and seeds. By using promoter analysis, the cis-elements of
UBiA genes were mainly in hormone signaling and stress responses. The qRT-PCR results showed that HaUBiA1 and HaUBiA5 responded strongly to abiotic stresses. Under ABA and MeJA treatments, HaUBiA1 significantly upregulated, while HaUBiA5 significantly decreased. Under cold stress, the expression of UBiA1 was significantly upregulated in the roots and stems, while UBiA5 expression was increased only in the leaves. Under anaerobic induction, UBiA1 and UBiA5 were both upregulated in the roots, stems and leaves. In summary, this study systematically classified the UBiA family and identified two abiotic stress candidate genes in the sunflower. It expands the understanding of the UBiA family and provides a theoretical basis for future abiotic stress studies in sunflowers.