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Abstract：
Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA-related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily.
摘要：
很少研究来自细菌和担子菌真菌的二萜环化酶。这里,我们提出了EriG的鉴定和验证，一个乌比超家族的成员,作为负责蘑菇猴头菌中氰化骨架环化的酶。使用EriG蛋白序列作为探针的基因组挖掘导致在细菌和真菌中发现了一个新的普遍存在的与UbiA相关的二萜环化酶家族。借助工程化的大肠杆菌菌株，我们成功地鉴定了来自细菌或担子菌真菌的7种新的二萜环化酶，并确定了其相应产物的结构。在此过程中产生了具有不寻常骨架的新二萜。这种新的二萜环化酶家族的发现提供了对UbiA超家族的新见解。
