背景:党参是一种常用的中药,有健脾补肺的作用,滋养血液和产生液体。此外,它也被用作重要的食品材料。
目的:本研究的目的是解释党参化学成分与药理作用之间的潜在相关性,并探索党参的生物活性标志物。
方法:闽县党参样品,以甘肃省不同加工方法为材料,对化合物进行UPLC-ESI-Q-TOF-MS/MS分析,建立UPLC指纹图谱。同时,研究了党参的造血和免疫功能,以获得相关的药理指标。然后,进行了UPLC指纹图谱中化学成分与药理作用的相关性分析。植物名称已确认到数据库“植物列表”(www.theplantlist.org)。
结果:根据典型相关分析的结果,色氨酸,注射器,汤仙须I,骨鼓群A,党参的造血和免疫功能相关的潜在生物活性标记物,可以推荐作为索引化合物。
结论:这项研究说明了化学成分与药理作用之间的潜在相关性,探索药理物质基础,为党参质量标准的提高奠定了基础。
BACKGROUND: Codonopsis Radix is a commonly used traditional Chinese medicine, and has the effect of strengthening spleen and tonifying lung, nourishing blood and engendering liquid. In addition, it is also used as important food materials.
OBJECTIVE: The aim of the study was to explain the underlying correlations between chemical constituents and pharmacological effects and explore the bioactive markers of Codonopsis Radix.
METHODS: Codonopsis Radix samples from Min county, Gansu province processed with different methods were taken as the materials, UPLC-ESI-Q-TOF-MS/MS analysis was conducted to identify the compounds and establish UPLC fingerprint. Meanwhile, hematopoietic and immunologic functions of Codonopsis Radix were investigated to obtain relevant pharmacological index. Then, the correlation analysis between chemical constituents in UPLC fingerprints and pharmacological effects was carried out. The plant name was confirmed to the database \"The Plant List\" (www.theplantlist.org).
RESULTS: According to the results of canonical correlation analysis, tryptophan, syringin, tangshenoside I, codonopyrrolidium A, lobetyolin and two unknown compounds might be the potential bioactive markers related to the hematopoietic and immunologic functions of Codonopsis Radix, which could be recommended as the index compounds.
CONCLUSIONS: This study illustrated the underlying correlations between chemical constituents and pharmacological effects, explored the pharmacological material basis, and could lay a foundation for the improvement of quality standard of Codonopsis Radix.