PDF(Pubmed)
Abstract：
Glycyrrhiza inflata Bat. produces a lot of licorice waste after water extraction, which also retains abundant total flavonoids (TFs) and licochalcone A. However, licorice residue is often wasted due to the lack of good utilization of resources in practical applications. This study first screened the optimal membrane pore size and resin type and then explored the mechanism and conditions of the adsorption of TFs on the resin. Then, different combinations and sequences of membrane and macroporous resin (MR) methods were investigated. It was found that using the membrane method for initial purification, followed by the MR method for further purification, yielded the best purification results. Next, response surface methodology was utilized to investigate the resin\'s dynamic desorption conditions for TFs. Finally, the TF purity increased from 32.9% to 78.2% (2.38-fold) after purification by a combined membrane-MR process; the purity of licochalcone A increased from 11.63 mg·g-1 to 22.70 mg·g-1 (1.95-fold). This study verified the feasibility of enriching TFs and licochalcone A from licorice residue using a membrane-MR coupling method. In addition, a quality-control method was established using a fingerprinting method on the basis of ultrahigh-performance liquid chromatography (UPLC) to ensure the stability of the enrichment process.
摘要：
膨胀甘草蝙蝠。提取水后会产生大量的甘草废物，它还保留了丰富的总黄酮(TFs)和甘草查尔酮A。然而，甘草渣在实际应用中由于缺乏良好的资源利用而经常被浪费。本研究首先筛选了最佳膜孔径和树脂类型，然后探索了TFs在树脂上的吸附机理和条件。然后，研究了膜和大孔树脂（MR）方法的不同组合和顺序。发现使用膜法进行初始纯化，然后用MR方法进一步纯化，产生了最好的纯化结果。接下来,利用响应面法研究了树脂对TFs的动态解吸条件。最后,通过膜-MR组合工艺纯化后，TF纯度从32.9％增加到78.2％（2.38倍）；甘草查尔酮A的纯度从11.63mg·g-1增加到22.70mg·g-1（1.95倍）。本研究验证了使用膜-MR耦合方法从甘草残渣中富集TFs和甘草查尔酮A的可行性。此外,在超高效液相色谱（UPLC）的基础上，使用指纹图谱方法建立了质量控制方法，以确保富集过程的稳定性。
