可以通过橄榄油部门的固体废物回收的生物活性化合物,如多酚,以其显著的抗氧化和抗菌活性而闻名,在营养食品中具有潜在的应用,化妆品,和食品工业。鉴于工业需求不断增长,多酚的市场价值越来越高,对使用超声波辅助提取(UAE)从橄榄渣中回收天然抗氧化剂化合物进行了系统研究。单因素参数,即,提取溶剂,时间,和固液比,进行了研究,评估总酚含量(TPC)的回收率和最终提取物的抗氧化活性。丙酮-水系统(50%v/v,20分钟,1:20gmL-1)表现出最高的总酚含量回收率(每克干提取物168.8±5.5mgGAE)。进一步评估橄榄果渣提取物(OPE)的抗氧化和抗菌活性。在DPPH中,ABTS,和CUPRAC,OPE表现出每克干提取物413.6±1.9、162.72±3.36和384.9±7.86mgTE的抗氧化能力,分别。抗菌研究表明,OPE对大肠杆菌的最低抑制活性(MIC)为2.5mgmL-1,对枯草芽孢杆菌的最低抑制活性为10mgmL-1。羟基酪醇和酪醇被确定为OPE的主要酚类化合物。此外,使用不同的OPE负载量(0.01-0.1%,w/v)。与对照膜相比,富含OPE的膜显示出剂量依赖性的抗自由基清除活性达到85.7±4.6%(ABTS),并且对枯草芽孢杆菌的抑制作用高达81%。对于含有OPE的膜,也观察到增加的UV光阻挡能力。这些结果表明,OPE是酚类化合物的有价值的来源,具有有希望的生物活性,可用于开发多功能食品包装材料。
Bioactive compounds that can be recovered by the solid wastes of the olive oil sector, such as polyphenols, are known for their significant antioxidant and antimicrobial activities with potential application in nutraceutical, cosmetic, and food industries. Given that industrial demands are growing, and the polyphenol market value is ever increasing, a systematic study on the recovery of natural antioxidant compounds from olive pomace using ultrasound-assisted extraction (UAE) was conducted. Single-factor parameters, i.e., the extraction solvent, time, and solid-to-liquid ratio, were investigated evaluating the total phenolic content (TPC) recovery and the antioxidant activity of the final extract. The acetone-water system (50% v/v, 20 min, 1:20 g mL-1) exhibited the highest total phenolic content recovery (168.8 ± 5.5 mg GAE per g of dry extract). The olive pomace extract (OPE) was further assessed for its antioxidant and antibacterial activities. In DPPH, ABTS, and CUPRAC, OPE exhibited an antioxidant capacity of 413.6 ± 1.9, 162.72 ± 3.36 and 384.9 ± 7.86 mg TE per g of dry extract, respectively. The antibacterial study showed that OPE attained a minimum inhibitory activity (MIC) of 2.5 mg mL-1 against E. coli and 10 mg mL-1 against B. subtilis. Hydroxytyrosol and
tyrosol were identified as the major phenolic compounds of OPE. Furthermore, active chitosan-polyvinyl alcohol (CHT/PVA) films were prepared using different OPE loadings (0.01-0.1%, w/v). OPE-enriched films showed a dose-dependent antiradical scavenging activity reaching 85.7 ± 4.6% (ABTS) and inhibition growth up to 81% against B. subtilis compared to the control film. Increased UV light barrier ability was also observed for the films containing OPE. These results indicate that OPE is a valuable source of phenolic compounds with promising biological activities that can be exploited for developing multifunctional food packaging materials.