背景:本研究旨在通过研究偏头痛急性发作痛觉调节途径中代谢产物的变化,探讨偏头痛大鼠导水管周围灰质(PAG)与双侧三叉神经尾核(TNC)不对称调节的相关性。
方法:30只雄性SD大鼠随机分为3组,control,模型组。然后,空白组腹腔注射超纯水,对照组注射生理盐水,模型组注射三硝酸甘油酯(GTN)。两小时后,分别去除PAG和双侧TNC,和PAG的代谢物浓度,左TNC,获得了右TNC。最后,比较了三种脑组织代谢产物的差异。
结果:rNAA的相对浓度,rGlu,rGln,rTau,PAG或双侧TNC中的rMI在组和部位之间存在交互作用。偏头痛大鼠三种脑组织rLac浓度升高,然而,LTNC和RTNC的rLac比PAG增加更多。此外,RTNC中rNAA和rGln的浓度增加,rGABA在RTNC中降低。
结论:PAG,LTNC和RTNC在调节急性偏头痛发作期间的疼痛中,LTNC和RTNC对疼痛的调节是不对称的。
BACKGROUND: The study was designed to explore the correlation of the asymmetric regulation between periaqueductal gray (PAG) and bilateral trigeminal nucleus caudalis (TNC) in migraine rats through studying the changes of metabolites in pain regulatory pathway of acute migraine attack.
METHODS: Thirty male Sprague-Dawley (SD) rats were randomly divided into three groups: blank, control, model groups. Then, blank group was intraperitoneally injected with ultrapure water, while control group injected with saline and model group injected with Glyceryl Trinitrate (GTN). Two hours later, PAG and bilateral TNC were removed respectively, and metabolite concentrations of PAG, Left-TNC, Right-TNC were obtained. Lastly, the differences of metabolite among three brain tissues were compared.
RESULTS: The relative concentrations of rNAA, rGlu, rGln, rTau, rMI in PAG or bilateral TNC had interaction effects between groups and sites. The concentration of rLac of three brain tissues increased in migraine rats, however, the rLac of LTNC and RTNC increased more than that of PAG. Besides, the concentrations of rNAA and rGln increased in RTNC, while rGABA decreased in RTNC.
CONCLUSIONS: There is correlation between PAG, LTNC and RTNC in regulation of pain during acute migraine attack, and the regulation of LTNC and RTNC on pain is asymmetric.