污染物对co足类的影响。它的分子反应是最少探索的,尽管它们在水生环境中的丰富和在无脊椎动物中占主导地位。在目前的调查中,刚性Dioithona,一种摆线浮游动物,用硒纳米颗粒(SeNPs)处理以确定相关的生化变化,并使用转录组学分析记录慢性暴露效应。结果发现,SeNPs具有急性毒性,致死剂量为140.9mg/L的50%。co足类的从头组装转录组包含81,814个转录本,随后对生物过程进行了注释(23,378),细胞成分(21,414),和分子功能(31,015)。表达的转录物与处理的样品的比较显示,总共186个转录物基因在硬毛虫处理(对照和SeNP)中差异表达。显著下调的基因编码DNA修复,DNA模板化DNA复制,DNA整合,氧化还原酶活性和跨膜转运。同样,在蛋白磷酸酶结合和膜复极化的调节中观察到显着的上调。了解SeNPs对co足类的影响不仅对水生生态系统健康而且对人类健康至关重要。因为这些生物在海洋食物网中发挥关键作用,最终影响人类食用的鱼。通过阐明SeNPs的分子反应和潜在的毒理学效应,这项研究为风险评估和监管政策提供了关键见解,确保海产品的安全,保护人类健康免受纳米颗粒污染的意外后果。
硬质Dioithona中的毒性分析是首次作为co足类模型用于分析营养级金属毒性的饮食固定。由于这种co足类是海洋生态系统中由鱼类和甲壳类幼虫喂养的主要浮游动物,对这种co足类的毒性分析将使我们对营养级食物转移有更多的了解。据我们所知,这是第一项选择为这种co足类动物构建从头转录组管道的研究,用硒纳米颗粒处理。这项工作的有效性可以进一步外推以评估其他金属纳米颗粒在该模型生物中的作用。尽管海洋生态系统中的硒毒性是一个既定的部门,通过我们的生化分析和组学相结合的方法,已经研究了硒在生殖适应性和分子变化中的毒性的坚实框架和综合见解。本研究选择在新的co足类模型和组学方法的意义上寻求可靠的替代方法来分析海洋生态系统中相关的金属纳米颗粒毒性。
The impact of contaminants on Copepod sp. and its molecular response is least explored, despite their abundance and dominance among invertebrates in aquatic environments. In the present investigation, Dioithona rigida, a cyclopoid zooplankton, was treated with selenium nanoparticles (SeNPs) to determine the associated biochemical changes, and the chronic exposure effects were recorded using transcriptomic analysis. It was found that, SeNPs were acutely toxic with a lethal dose 50% of 140.9 mg/L. The de novo assembled transcriptome of the copepod comprised 81,814 transcripts, which underwent subsequent annotations to biological processes (23,378), cellular components (21,414), and molecular functions (31,015). Comparison of the expressed transcripts against the treated sample showed that a total of 186 transcript genes were differentially expressed among the D. rigida treatments (control and SeNPs). The significant downregulated genes are coding for DNA repair, DNA-templated DNA replication, DNA integration, oxidoreductase activity and transmembrane transport. Similarly, significant upregulations were observed in protein phosphatase binding and regulation of membrane repolarization. Understanding the impact of SeNPs on copepods is crucial not only for aquatic ecosystem health but also for human health, as these organisms play a key role in marine food webs, ultimately affecting the fish consumed by humans. By elucidating the molecular responses and potential toxicological effects of SeNPs, this study provides key insights for risk assessments and regulatory policies, ensuring the safety of seafood and protecting human health from the unintended consequences of nanoparticle pollution.
The toxicity analysis in Dioithona rigida is the first of its kind as a copepod model for analysis on dietary fixation of metal toxicity at the trophic level. Since this copepod is a major zooplankton fed by fish and crustacean larvae in marine ecosystems, the toxicity analysis on this copepod will give us more insights of the trophic-level food transfer. As far as our knowledge, this is the first study that opted to construct the de novo transcriptomic pipeline for this copepod, treated with selenium nanoparticles. The effectiveness of this work may be further extrapolated to assess the effect of other metal nanoparticles in this model organism. Although the selenium toxicity in marine ecosystem is an established sector, through our combined approach of biochemical analysis and omics approach, the solid framework and comprehensive insight of the selenium toxicity in reproductive fitness and molecular changes has been studied. This study chose to seek a reliable alternative in the sense of new copepod model and omics approach to analyse the relevant metal nanoparticle toxicity in the marine ecosystem.