巴尔通体是一种细胞内寄生的人畜共患病原体,可感染动物并引起多种人类疾病。本研究调查了云南省小型哺乳动物的巴尔通体流行情况。中国,关注组织嗜性。共抽取了十三个物种的333只小型哺乳动物,三个命令,四个家庭,鹤庆和贡山县的四个属。常规PCR和实时定量PCR(qPCR)用于检测和定量,然后对获得的DNA序列进行生物信息学分析。结果显示31.5%的检出率,不同物种。值得注意的是,ApodemusChevrieri,Eothenomyseleusis,Niviventer饱食者,Rattustanezumi,短尾蛇,Anourosorexsquamipes,OchotonaThibetana的感染率为44.4%,27.7%,100.0%,6.3%,60.0%,23.5%,和22.2%,分别。遗传分析确定了三十,十,和基于ssrA的五种菌株,rpoB,和gltA基因,核苷酸同一性范围从92.1%到100.0%。巴尔通体菌株被分配给格拉哈姆氏杆菌,B.rochalimae,B.仙台,B.koshimizu,B.phoceensis,B.taylorii,和一个新的物种鉴定在菊花leucops(GS136)。对巴尔通体自然感染的不同组织的分析显示,不同组织的拷贝数不同,脾脏组织负荷最高。这些发现强调了云南省巴尔通菌的多样性物种和寄主范围,突出了在自然感染小型哺乳动物组织的巴尔通体物种中广泛的组织嗜性的存在。
Bartonella is an intracellular parasitic zoonotic pathogen that can infect animals and cause a variety of human diseases. This study investigates Bartonella prevalence in small mammals in Yunnan Province, China, focusing on tissue tropism. A total of 333 small mammals were sampled from thirteen species, three orders, four families, and four genera in Heqing and Gongshan Counties. Conventional PCR and real-time quantitative PCR (qPCR) were utilized for detection and quantification, followed by bioinformatic analysis of obtained DNA sequences. Results show a 31.5% detection rate, varying across species. Notably, Apodemus chevrieri, Eothenomys eleusis, Niviventer fulvescens, Rattus tanezumi, Episoriculus leucops, Anourosorex squamipes, and Ochotona Thibetana exhibited infection rates of 44.4%, 27.7%, 100.0%, 6.3%, 60.0%, 23.5%, and 22.2%, respectively. Genetic analysis identified thirty, ten, and five strains based on ssrA, rpoB, and gltA genes, with nucleotide identities ranging from 92.1% to 100.0%. Bartonella strains were assigned to B. grahamii, B. rochalimae, B. sendai, B. koshimizu, B. phoceensis, B. taylorii, and a new species identified in Episoriculus leucops (GS136). Analysis of the different tissues naturally infected by Bartonella species revealed varied copy numbers across different tissues, with the highest load in spleen tissue. These findings underscore Bartonella\'s diverse species and host range in Yunnan Province, highlighting the presence of extensive tissue tropism in Bartonella species naturally infecting small mammalian tissues.