PDF(Pubmed)
Abstract：
Here, we report the discovery of two viruses associated with a disease characterized by severe diarrhea on a large-scale goat farm in Jilin province. Electron Microscopy observations revealed two kinds of virus particles with the sizes of 150-210 nm and 20-30 nm, respectively. Detection of 276 fecal specimens from the diseased herds showed the extensive infection of peste des petits ruminants virus (63.77%, 176/276) and caprine enterovirus (76.81%, 212/276), with a co-infection rate of 57.97% (160/276). These results were partially validated with RT-PCR, where all five PPRV-positive and CEV-positive specimens yielded the expected size of fragments, respectively, while no fragments were amplified from PPRV-negative and CEV-negative specimens. Moreover, corresponding PPRV and CEV fragments were amplified in PPRV and CEV double-positive specimens. Histopathological examinations revealed severe microscopic lesions such as degeneration, necrosis, and detachment of epithelial cells in the bronchioles and intestine. An immunohistochemistry assay detected PPRV antigens in bronchioles, cartilage tissue, intestine, and lymph nodes. Simultaneously, caprine enterovirus antigens were detected in lung, kidney, and intestinal tissues from the goats infected by the peste des petits ruminants virus. These results demonstrated the co-infection of peste des petits ruminants virus with caprine enterovirus in goats, revealing the tissue tropism for these two viruses, thus laying a basis for the future diagnosis, prevention, and epidemiological survey for these two virus infections.
摘要：
这里,我们报告了在吉林省的一个大型山羊养殖场发现的两种病毒与一种以严重腹泻为特征的疾病有关。电子显微镜观察发现两种大小为150-210nm和20-30nm的病毒颗粒，分别。从患病牛群中检测到276个粪便标本，显示小反刍动物病毒的广泛感染（63.77％，176/276）和山羊肠道病毒（76.81%，212/276），合并感染率为57.97%(160/276)。这些结果用RT-PCR部分验证，所有五个PPRV阳性和CEV阳性标本都产生了预期大小的碎片，分别,而PPRV阴性和CEV阴性标本中没有扩增片段。此外，在PPRV和CEV双阳性标本中扩增了相应的PPRV和CEV片段。组织病理学检查显示严重的微观病变，如变性，坏死,细支气管和肠道上皮细胞的脱离。免疫组织化学检测在细支气管中检测到PPRV抗原，软骨组织,肠,和淋巴结。同时,在肺中检测到山羊肠道病毒抗原，肾,和受小反刍动物病毒感染的山羊的肠道组织。这些结果表明小反刍动物病毒与山羊肠道病毒在山羊中共同感染，揭示了这两种病毒的组织嗜性，从而为未来的诊断奠定基础，预防,以及这两种病毒感染的流行病学调查。
