Standard bacteriological examinations, which involve culturing microorganisms at 37 °C, are commonly used in clinical practice for diagnosing infectious diseases. However, the growth temperature of microorganisms on the ocular surface (OS) during infectious keratitis (IK) may not coincide with the laboratory standard, which is due to the characteristic features of heat exchange in the eye.
OBJECTIVE: This exploratory study examines the distribution and properties of OS microorganisms isolated under different temperature cultivation conditions in patients with IK and healthy volunteers without ophthalmic pathology.
METHODS: Fifteen participants were divided into two groups. Group 1 (n=10) consisted of patients with signs of unilateral infectious keratitis, while group 2 (n=5) served as the control group. A novel microbiological method was employed to isolate pure cultures of microorganisms. This method involved cultivating microorganisms at two temperature regimes (37 °C and 24 °C) and subsequently identifying them using biochemical, immunological, and physicochemical techniques, including mass spectrometry. Scanning electron microscopy (SEM) with lanthanide staining used as the reference method. The temperature status of the ocular surface was assessed using non-contact infrared thermography.
RESULTS: The study demonstrated the presence of psychrotolerant microorganisms on the ocular surface, which exhibited growth at a relatively low temperature of 24 °C. These psychrotolerant microorganisms were found to be isolated from the ocular surface displaying signs of temperature dysregulation. Among such microorganisms are Acinetobacter lwoffii, Achromobacter xylosoxidans, Bacillus licheniformis, Enterococcus faecalis, Klebsiella oxytoca, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas luteola, Streptococcus spp.
CONCLUSIONS: When identifying the causative agent of infectious keratitis, it is crucial to consider the divergence of growth temperature of ocular surface microorganisms. The presence of psychrotolerant microorganisms on the ocular surface, which can effectively grow at room temperature, should be taken into account, especially in cases of temperature dysregulation.
В широкой клинической практике для диагностики инфекционных заболеваний обычно используют стандартное бактериологическое исследование, предусматривающее выделение микроорганизмов при 37 °C. Однако температурный оптимум роста микроорганизмов на глазной поверхности (ГП) при инфекционных кератитах (ИК) может не совпадать с лабораторным, что обусловлено особенностями теплообмена глаза.
UNASSIGNED: В рамках поискового исследования предполагалось изучить состав и свойства микроорганизмов ГП, выделенных в различных температурных условиях культивирования, у пациентов с ИК и здоровых добровольцев без офтальмопатологии.
UNASSIGNED: В исследовании приняли участие 15 человек, разделенных на две группы: 1-я группа (n=10) — пациенты с признаками одностороннего инфекционного кератита; 2-я группа (n=5) — условная норма. Для идентификации микроорганизмов применяли оригинальный микробиологический метод — выделение чистых культур микроорганизмов на питательных средах при двух температурных режимах (37 °C и 24 °C) с их последующей идентификацией с использованием биохимических, иммунологических, физико-химических методов, включая масс-спектрометрические. В качестве метода визуализации проводили сканирующую электронную микроскопию импрессионной пробы с ГП. Для исследования температурного статуса ГП использовали бесконтактную инфракрасную термографию.
UNASSIGNED: Продемонстрировано, что на ГП существуют психротолерантные микроорганизмы, которые эффективно растут при относительно низкой температуре (24 °C). Показано, что психротолерантные микроорганизмы, как правило, выделяются с ГП при ИК с признаками температурной дисрегуляции. Установлено, что среди таких микроорганизмов обнаруживаются Acinetobacter lwoffii, Achromobacter xylosoxidans, Bacillus licheniformis, Enterococcus faecalis, Klebsiella oxytoca, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas luteola, Streptococcus spp.
UNASSIGNED: При установлении возбудителя ИК следует принимать во внимание то, что при температурной дисрегуляции на ГП могут существовать психротолерантные микроорганизмы, которые эффективно растут при комнатной температуре.

Joint hypermobility syndromes, particularly chronic pain associated with this condition, including Hypermobile Ehlers-Danlos Syndrome (hEDS) and Hypermobility Spectrum Disorders (HSD), present diagnostic challenges due to their multifactorial origins and remain poorly understood from biomechanical and genomic-molecular perspectives. Recent diagnostic guidelines have differentiated hEDS, HSD, and benign joint hypermobility, providing a more objective diagnostic framework. However, incorrect diagnoses and underdiagnoses persist, leading to prolonged journeys for affected individuals. Musculoskeletal manifestations, chronic pain, dysautonomia, and gastrointestinal symptoms illustrate the multifactorial impact of these conditions, affecting both the physical and emotional well-being of affected individuals. Infrared thermography (IRT) emerges as a promising tool for joint assessment, especially in detecting inflammatory processes. Thermal distribution patterns offer valuable insights into joint dysfunctions, although the direct correlation between pain and inflammation remains challenging. The prevalence of neuropathies among hypermobile individuals accentuates the discordance between pain perception and thermographic findings, further complicating diagnosis and management. Despite its potential, the clinical integration of IRT faces challenges, with conflicting evidence hindering its adoption. However, studies demonstrate objective temperature disparities between healthy and diseased joints, especially under dynamic thermography, suggesting its potential utility in clinical practice. Future research focused on refining diagnostic criteria and elucidating the underlying mechanisms of hypermobility syndromes will be essential to improve diagnostic accuracy and enhance patient care in this complex and multidimensional context.

UNASSIGNED: Infrared thermography (IT) is a non-invasive real-time imaging technique with potential application in different areas of neurosurgery. Despite technological advances in the field, intraoperative IT (IIT) has been an underestimated tool with scarce reports on its usefulness during intracranial tumor resection. We aimed to evaluate the usefulness of high-resolution IIT with static and dynamic thermographic maps for transdural lesion localization, and diagnosis, to assess the extent of resection, and the occurrence of perioperative acute ischemia.
UNASSIGNED: In a prospective study, 15 patients affected by intracranial tumors (six gliomas, four meningiomas, and five brain metastases) were examined with a high-resolution thermographic camera after craniotomy, after dural opening, and at the end of tumor resection.
UNASSIGNED: Tumors were transdurally located with 93.3% sensitivity and 100% specificity (p < 0.00001), as well as cortical arteries and veins. Gliomas were consistently hypothermic, while metastases and meningiomas exhibited highly variable thermographic maps on static (p = 0.055) and dynamic (p = 0.015) imaging. Residual tumors revealed non-specific static but characteristic dynamic thermographic maps. Ischemic injuries were significantly hypothermic (p < 0.001).
UNASSIGNED: High-resolution IIT is a non-invasive alternative intraoperative imaging method for lesion localization, diagnosis, assessing the extent of tumor resection, and identifying acute ischemia changes with static and dynamic thermographic maps.

Glass fiber-reinforced polymer (GFRP) laminates are used in many applications because of their availability, high mechanical properties, and cost-effectiveness. Fiber defects in the form of waviness or wrinkles can occur during the production of multilayered laminates. When curved laminates of significant thickness are produced, the likelihood of such defects increases. Studies have confirmed that fiber deformation during manufacture leads to a reduction in the mechanical properties of laminates. Therefore, early detection of such defects is essential. The main part of this paper deals with research into the possibility of using active infrared thermography to detect wrinkles in curved multilayered GFRP laminates. The size of the artificial wrinkles was assessed by analyzing scans and microimages. The shape deformations of the samples were evaluated by comparing the samples with the mold and the assumed nominal shape. The influence of the out-of-autoclave manufacturing process on the reduction in wrinkles formed without significantly affecting the internal structure of the laminate is presented in this work. This research demonstrated the ability to detect wrinkles in thick curved laminates using active infrared thermography. However, it also showed how the interpretation of the thermographic results is affected by the curvature of the structure, the lack of uniform heating, and the configuration of the thermographic setup.

暂无摘要。

BACKGROUND: Acupuncture is a method for treating tic disorder. However, there is a lack of sufficient clinical objective basis in regards of its treatment efficacy. Indeed, there are structural abnormalities present in energy metabolism and infrared thermography in children with tic disorder. Therefore, this study proposes a clinical trial scheme to explore the possible mechanism of acupuncture in treating tic disorder.
METHODS: This randomized controlled trial will recruit a total of 90 children, in which they will be divided into non-intervention group and intervention group. The non-intervention group consists of 30 healthy children while the intervention group consists of 60 children with tic disorder. The intervention group will be randomly allocated into either the treatment group or the control group, with 30 children randomly assigned in each group. Children either received acupuncture treatment and behavioral therapy (treatment group) or sham acupuncture treatment and behavioral therapy (control group), 3 treatment sessions per week for a period of 12 weeks, with a total of 36 treatment sessions. Outcome measures include YGTSS, urinary and fecal metabolomics, infrared thermography of body surface including governor vessel. For the intervention group, these outcome measures will be collected at the baseline and 90th day prior to intervention. Whereas for the non-intervention group, outcome measures (excluding YGTSS) will be collected at the baseline.
CONCLUSIONS: The main outcome will be to observe the changes of the severity of tic condition, the secondary outcome will be to observe the changes of structural characteristic of infrared thermography of body surface/acupoints along the governor vessel and to evaluate the changes of urinary and fecal metabolomics at the end of the treatment, so as to analyze the relationship between them and to provide further knowledge in understanding the possible mechanism of acupuncture in improving the clinical symptoms via regulating and restoring the body metabolomics network, which in future it can develop as a set of clinical guideline (diagnosis, treatment, assessment, prognosis) in treating tic disorder. ChiCTR2300075188(Chinese Clinical Trial Registry, http://www.chictr.org.cn , registered on 29 August 2023).

Maintaining high-quality welded connections is crucial in many industries. One of the challenges is assessing the mechanical properties of a joint during its production phase. Currently, in industrial practice, this occurs through NDT (non-destructive testing) conducted after the production process. This article proposes the use of a virtual sensor, which, based on temperature distributions observed on the joint surface during the welding process, allows for the determination of hardness distribution across the cross-section of a joint. Welding trials were conducted with temperature recording, hardness measurements were taken, and then, neural networks with different hyperparameters were tested and evaluated. As a basis for developing a virtual sensor, LSTM networks were utilized, which can be applied to time series prediction, as in the analyzed case of hardness value sequences across the cross-section of a welded joint. Through the analysis of the obtained results, it was determined that the developed virtual sensor can be applied to predict global temperature changes in the weld area, in terms of both its value and geometry changes, with the mean average error being less than 20 HV (mean for model ~35 HV). However, in its current form, predicting local hardness disturbances resulting from process instabilities and defects is not feasible.

The aim of this study was to assess, through a systematic review, the status of infrared thermography (IRT) as a diagnostic tool for skin neoplasms of the head and neck region and in order to validate its effectiveness in differentiating benign and malignant lesions. A search was carried out in the LILACS, PubMed/MEDLINE, SCOPUS, Web of Science and EMBASE databases including studies published between 2004 and 2024, written in the Latin-Roman alphabet. Accuracy studies with patients aged 18 years or over presenting benign and malignant lesions in the head and neck region that evaluated the performance of IRT in differentiating these lesions were included. Lesions of mesenchymal origin and studies that did not mention histopathological diagnosis were excluded. The systematic review protocol was registered in the PROSPERO database (CRD42023416079). Reviewers independently analyzed titles, abstracts, and full-texts. After extracting data, the risk of bias of the selected studies was assessed using the QUADAS - 2 tool. Results were narratively synthesized and the certainty of evidence was measured using the GRADE approach. The search resulted in 1,587 records and three studies were included. Only one of the assessed studies used static IRT, while the other two studies used cold thermal stress. All studies had an uncertain risk of bias. In general, studies have shown wide variation in the accuracy of IRT for differentiating between malignant and benign lesions, with a low level of certainty in the evidence for both specificity and sensitivity.

BACKGROUND: The Morel-Lavallee lesion (MLL) is a closed, degloving soft-tissue injury, wherein the skin and subcutaneous tissue are separated from the underlying fascia. This syndrome causes disruption of wound healing. Infrared thermography is a noninvasive and pain-free tool that can be used to evaluate scar and the influence of osteopathic manipulative treatment.
OBJECTIVE: To evaluate the influence of post-operative osteopathic manipulative treatment (OMT) of Morel-Lavallee lesions (MLL).
METHODS: During four osteopathic sessions on one volunteer patient, 28-year-old male, resulting in MLL of the left knee after motorcycle accident. The effects of OMT were assessed using an infrared thermal imaging camera and qualitative palpation examination of osteopathic dysfunction, scored on a scale of 1-4.
RESULTS: and discussion: Both scar and peri-scar area temperatures increased after OMT. The difference in temperature between the scar and the peri-scar area decreased after OMT. Increase in temperature was greater when the OMT was applied around the scar than when applied at a distance from the scar site. The palpation score for dysfunction of the MLL scar site decreased from 4/4 to 2/4 after the final session.
CONCLUSIONS: Several OMT sessions focusing on the MLL scar site appear necessary to obtain noteworthy results. OMT improved mobility and increased the temperature of the scar and the peri-scar area.

BACKGROUND: The aim of this study was to determine the level of participation in the training of the athlete who applied to the clinic with pain by infrared thermography. Symptoms of sartorius muscle (SM) injury are like rectus femoris injuries.
UNASSIGNED: Grade I SM injury of a 23-year-old male football player was determined by thermographic diagnosis. Taking a resting thermal image before the training of the player reported a pain in the upper thigh region.
RESULTS: Since both legs were equally loaded, in accordance with the method we developed, the thermal image was taken again after a 10-min cycling program with 30-40% resistance. The heat maps of legs seen in the pre- and post-training images were analyzed. There was no asymmetrical finding indicating injury in the resting thermographic evaluation, but asymmetric findings showing the injury in the region of SM were obtained in the repeated thermographic imaging after the 10-min cycling program. Grade I SM injury was detected by MRI afterwards.
CONCLUSIONS: Even if there is no sign of asymmetry in the resting thermography of football players having signs of pain, the injured muscle should be provoked with a safe exercise program and the thermal image should be retaken.