人脂肪干细胞(hASCs)在再生医学中发挥着重要作用。
探索Rg1促进hASCs增殖和成脂分化的机制在再生医学研究中具有重要意义。
观察人参皂苷Rg1促进hASCs增殖和成脂分化的作用,建立不同浓度的Rg1培养基,并使用细胞计数试剂盒-8(CCK-8)测定法进行测试。油红O染色,茜素红,还有AlcianBlue.与对照相比,通过DEG分析筛选差异表达基因(DEGs),这是在基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径富集分析中进行的。为了探索mRNA之间的关系,长链非编码RNA(lncRNA)和microRNA(miRNA),我们构建了一个竞争内源性RNA(ceRNA)网络。
在这项研究中,观察到Rg1促进hASC的增殖和成脂分化。此外,富集的BP和KEGG途径可能参与促进过程,其中FXR1和Lnc-GAS5-AS1被发现是调节因子。该调控网络提示Rg1可通过FXR1和Lnc-GAS5-AS1调节脂肪细胞因子信号通路和IL-17信号通路,其机制包括促进Rg1对hASCs增殖和成脂分化的作用。
构建了与Rg1促进能力相关的综合转录调控网络,揭示了Rg1促进hASCs增殖和成脂分化的机制。本研究为hASCs的功能优化提供了理论依据。
Human adipose-derived stem cells (hASCs) play an important role in regenerative medicine.
Exploring the mechanism of Rg1 in the promotion of the proliferation and adipogenic differentiation of hASCs is important in regenerative medicine research.
To observe ginsenoside Rg1 in promoting the proliferation and adipogenic differentiation of hASCs, Rg1 medium at different concentrations was established and tested using the cell counting kit-8 (CCK-8) assay, oil red O staining, alizarin red, and alcian blue. Compared to the control, differentially expressed genes (DEGs) were screened via DEG analysis, which was carried out in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. To explore the relationship among mRNA, long non-coding RNA (lncRNA) and microRNA (miRNA), we constructed a competing endogenous RNA (ceRNA) network.
In this study, Rg1 was observed to promote the proliferation and adipogenic differentiation of hASCs. Additionally, enriched BPs and KEGG pathways may be involved in the promotion process, where FXR1 and Lnc-GAS5-AS1 were found to be regulatory factors. The regulatory network suggested that Rg1 could regulate the adipocytokine signaling pathway and IL-17 signaling pathway via FXR1 and Lnc-GAS5-AS1, which served as the mechanism encompassing the promotion of Rg1 on the proliferation and adipogenic differentiation of hASCs.
A comprehensive transcriptional regulatory network related to the promotion ability of Rg1 was constructed, revealing mechanisms regarding Rg1\'s promotion of the proliferation and adipogenic differentiation of hASCs. The present study provides a theoretical basis for optimizing the function of hASCs.