目的:研究三角塔林叶提取物甲醇对血液学参数的影响,酶和非酶抗氧化剂状态,和标准实验室喂养的Wistar大鼠的血脂,或者富含2%胆固醇的饮食.
方法:Wistar大鼠(180-210g)分为六组,每组六只动物(雄性),每天饲喂2%富含胆固醇的饮食,并口服0.9%盐水或三角塔利坦提取物(250、500和1000mg/kg体重),共八周。脂质轮廓,脂质过氧化(MDA),血液学参数,及其功能指标和血清抗氧化酶(过氧化氢酶,谷胱甘肽-S-转移酶,在正常和饮食诱导的高胆固醇血症提取物治疗的大鼠中评估了超氧化物歧化酶)活性和谷胱甘肽状态,并与每bwt标准药物吉非贝齐100mg/kg治疗的大鼠进行了比较。
结果:血脂(总甘油酯,总胆固醇,低密度脂蛋白,和极低密度脂蛋白),与正常饮食的动物相比,在高胆固醇血症大鼠的血清中观察到MDA和酶和非酶抗氧化剂状态的降低(P<0.05)以及血液学参数的改变。三角石或吉非罗齐的甲醇叶提取物的共同给药显著(P<0.05)恢复了升高的血清脂质谱,MDA,血液学参数紊乱接近正常。该提取物还可以防止高胆固醇血症诱导的酶促和非酶促抗氧化剂状态降低。植物提取物的活性是剂量依赖性的(250、500和1000mg/kg),它与标准药物吉非贝齐相比是有利的。
结论:本研究表明,三角塔利金提取物可能对高胆固醇血症引起的脂质改变具有保护作用,氧化应激,并改善抗氧化防御系统和造血功能的状况。
结论:脂质分布升高(总甘油酯,总胆固醇,低密度脂蛋白,和极低密度脂蛋白),脂质过氧化(MDA),与正常饮食的动物相比,在高胆固醇血症大鼠的血清中观察到酶促和非酶促抗氧化剂状态的降低以及血液学参数的改变。MDA,血液学参数紊乱接近正常。该提取物还可以防止高胆固醇血症诱导的酶促和非酶促抗氧化剂状态降低。植物提取物的活性是剂量依赖性的,与标准药物吉非贝齐相比具有优势。使用的缩写:脂质过氧化(MDA),(过氧化氢酶(CAT),谷胱甘肽-S-转移酶(GST),超氧化物歧化酶(SOD),谷胱甘肽(GSH),血小板指数(PLT),红细胞(RBC),包装电池体积(PVC),平均红细胞血红蛋白(MCH),平均红细胞血红蛋白浓度(MCHC),总甘油酯(TG),极低密度脂蛋白(VLDL),总胆固醇(TC),低密度脂蛋白(LDL),高密度脂蛋白(HDL)和3-羟基-3-甲基-戊二酰基-CoA还原酶(HMG-CoA)。
OBJECTIVE: To investigate the effect of methanolic leaf extract of Talinum triangulare on hematological parameters, enzymatic and non-enzymatic antioxidant status, and serum lipid in Wistar rats fed standard laboratory, or 2% cholesterol-enrich diet.
METHODS: Wistar rats (180-210g) divided into six groups of six animals (males) each were fed 2% cholesterol-enriched diet and orally treated with 0.9% saline or extract of Talinum triangulare (250, 500, and 1000 mg/kg per body weight) daily for eight weeks. Lipid profile, lipid peroxidation (MDA), hematological parameters, and their functional indices and serum antioxidant enzymes (catalase, glutathione -S-transferase, and superoxide dismutase) activities and glutathione status were assessed in normal and diet-induced hypercholesterolemic extract treated rats and compared with the rats treated with 100 mg/kg per bwt standard drug gemfibrozil.
RESULTS: A significant (P < 0.05) increase in lipid profile (total glyceride, total cholestrol, low-density lipoprotein, and very low-density lipoprotein), MDA and reduction (P < 0.05) in enzymatic and nonenzymatic antioxidant status coupled with alterations in hematological parameters was observed in the serum of hypercholesterolemic rats when compared with animals on a normal diet. Coadministration of methanolic leaf extracts of Talinum triangulare or gemfibrozil significantly (P < 0.05) restored the elevated serum lipid profile, MDA, and the deranged hematological parameters to near normal. The extract also protected against hypercholesterolemic-induced diminished enzymatic and nonenzymatic antioxidant status. The activities of the plant extract are dose (250, 500, and 1000 mg/kg) dependent and it compared favorably with the standard drug gemfibrozil.
CONCLUSIONS: The present study suggested that the extract of Talinum triangulare might protect against hypercholesterolemic-induced altered lipid profiles, oxidative stress, and also improve the status of antioxidant defense system and hematopoiesis.
CONCLUSIONS: Elevated lipid profile (total glyceride, total cholestrol, low-density lipoprotein, and very low-density lipoprotein), lipid peroxidation (MDA), and reduced enzymatic and nonenzymatic antioxidant status coupled with alterations in hematological parameters was observed in the serum of hypercholesterolemic rats when compared with animals on a normal dietCoadministration of methanolic leaf extracts of Talinum triangulare significantly (P < 0.05) restored the elevated serum lipid profile, MDA, and the deranged hematological parameters to near normal.The extract also protected against hypercholesterolemic-induced diminished enzymatic and bnonenzymatic antioxidant status.The activities of the plant extract was dose-dependent and it compared favorably with the standard drug gemfibrozil. Abbreviations used: Lipid peroxidation (MDA), (catalase (CAT), glutathione-S-transferase (GST), superoxide dismutase (SOD), glutathione (GSH), Thrombocytes indices (PLT), Red blood cell (RBC), Packed cell volume (PVC), Mean corpuscular hemoglobin(MCH), Mean corpuscular hemoglobin concentration (MCHC), Total glyceride (TG), Very low density lipoprotein (VLDL), Total cholesterol (TC), Low density lipoprotein (LDL), High density lipoprotein (HDL) and 3-Hydroxy-3-methyl-glutaryl-CoA reductase(HMG-CoA).