疫霉,有203种,是全世界农业中非常重要的一个属。在这里,我们提供了在线资源“IDphy”(链接),以促进尽可能使用原始描述中的类型标本正确鉴定疫霉物种。IDphy强调对美国具有高经济影响和监管关注的物种。IDphy展示了截至2018年5月描述的161种可培养物种的交互式Lucid键和表格键,其中包括141种前类型和20个经过身份验证的标本。IDphy包含用于形态学和分子表征的SOP,以及词汇表,图片库,和许多链接。161份情况说明书中的每一份都包括对命名法的访问,形态和分子特征,包括ITSrDNA的序列,COI(条形码基因),YPT1,β-桶,EF1α,L10、HSP90等基因。IDphy包含一个创新的计算机BLAST和使用NCBI的系统发育序列分析。IDphy移动应用程序,2021年8月发布(适用于Android或iOS免费)(Link)允许用户将Lucid密钥带入实验室。IDphy是第一个基于植物病原体实施的类型的在线识别工具。在这份手稿中,我们还包括在IDphy出版后描述的21个新物种和一个杂种的信息,国际草药和文化收藏品中的类型和类型标本的状态,以及GenBank的基因组状态(目前有153个基因组装配,对应于42个描述的物种,包括16个类型)。IDphy在线资源的模型有效性和该手稿的内容可以激发其他研究人员为其他重要的植物病原体组开发其他识别工具。
Phytophthora, with 203 species, is a genus of high importance in agriculture worldwide. Here, we present the online resource \"IDphy\", developed to facilitate the correct identification of species of Phytophthora using the type specimens from the original descriptions wherever possible. IDphy emphasizes species of high economic impact and regulatory concern for the United States. IDphy presents an interactive Lucid key and a tabular key for 161 culturable species described as of May 2018, including 141 ex-types and 20 well-authenticated specimens. IDphy contains standard operating procedures for morphological and molecular characterization, as well as a glossary, image gallery, and numerous links. Each of the 161 factsheets includes access to nomenclature and morphological and molecular features, including sequences of the internal transcribed spacer ribosomal DNA, cytochrome C oxidase subunit I (barcoding genes), YPT1, β-tubulin, elongation factor 1a, L10, heat shock protein 90, and other genes. IDphy contains an innovative in silico BLAST and phylogenetic sequence analysis using NCBI. The IDphy mobile app, released in August 2021 (free for Android or iOS), allows users to take the Lucid key into the laboratory. IDphy is the first online identification tool based on the ex-types implemented for plant pathogens. In this article, we also include information for 21 new species and one hybrid described after the publication of IDphy, the status of the specimens of the types and ex-types at international herbaria and culture collections, and the status of genomes at the GenBank (currently 153 genome assemblies which correspond to 42 described species, including 16 ex-types). The effectiveness of the IDphy online resource and the content of this article could inspire other researchers to develop additional identification tools for other important groups of plant pathogens.