肉型鸡的骨骼完整性受到许多因素的影响,包括快速生长速度,营养和遗传学。探讨骨和矿物质代谢的遗传基础,在两个肉型鸡品系之间的杂交中进行了QTL检测研究,这些肉型鸡具有高(D)或低(D-)的消化效率。胫骨尺寸(长度,直径,在3周龄时测定体积)和灰分含量以及磷(P)保留和血浆浓度。估计了这些性状的遗传力及其与消化效率的遗传相关性。使用3379个SNP标记进行QTL定位研究。胫骨尺寸,体重,灰分含量和断裂强度具有高度遗传性(0.42至0.61)。相对胫骨直径和体积以及P保留与消化效率呈强烈正相关(0.57至0.80)。总共鉴定了35个QTL(胫骨重量为9个,胫骨尺寸为13,5骨强度,5为骨矿化,血浆P浓度为2,P保留为1)。六个QTL是全基因组显著的,和3个胫骨相对体积的QTL,6号染色体上的重量和灰分重量是固定的,来自D系的阳性等位基因.对于18号染色体上的灰分含量和26号染色体上的相对胫骨长度的两个QTL,置信区间小到足以识别潜在的候选基因。这些发现支持多个遗传基因座控制骨和矿物质代谢的证据。候选基因的识别可能为理解骨骼调节提供新的视角,甚至超越了鸟类。
Skeletal integrity in meat-type chickens is affected by many factors including rapid growth rate, nutrition and genetics. To investigate the genetic basis of bone and mineral metabolism, a QTL detection study was conducted in an intercross between two lines of meat-type chickens divergently selected for their high (D +) or low (D -) digestive efficiency. Tibia size (length, diameter, volume) and ash content were determined at 3 weeks of age as well as phosphorus (P) retention and plasma concentration. Heritability of these traits and their genetic correlations with digestive efficiency were estimated. A QTL mapping study was performed using 3379 SNP markers. Tibia size, weight, ash content and breaking strength were highly heritable (0.42 to 0.61). Relative tibia diameter and volume as well as P retention were strongly and positively genetically correlated with digestive efficiency (0.57 to 0.80). A total of 35 QTL were identified (9 for tibia weight, 13 for tibia size, 5 for bone strength, 5 for bone mineralization, 2 for plasma P concentration and 1 for P retention). Six QTL were genome-wide significant, and 3 QTL for tibia relative volume, weight and ash weight on chromosome 6 were fixed, the positive allele coming from the D-line. For two QTL for ash content on chromosome 18 and relative tibia length on chromosome 26, the confidence intervals were small enough to identify potential candidate genes. These findings support the evidence of multiple genetic loci controlling bone and mineral metabolism. The identification of candidate genes may provide new perspectives in the understanding of bone regulation, even beyond avian species.