苦参碱通过维持 AMPK α / UCP2 通路减轻多柔比星诱导的心脏毒性中的氧化应激和心肌细胞凋亡。
关键词: 4-HNE, 4-hydroxynonenal ACC, acetyl-CoA carboxylase AMPKα AMPKα, 5′-AMP-activated protein kinase α ANOVA, analysis of variance Apoptosis BAX, BCL-2-associated X protein BCA, bicinchoninic acid BCL-2, B-cell lymphoma 2 C-caspase 3, cleaved-caspase3 CCK-8, cell counting kit 8 CK-MB, creatine kinase isoenzymes DCFH-DA, 2′,7′-dichlorodihydrofluorescein diacetate DHE, dihydroethidium DMEM, Dulbecco׳s modified Eagle׳s medium DOX, doxorubicin FBS, fetal bovine serum FS, fractional shortening GAPDH, glyceraldehyde 3-phosphate dehydrogenase HW, heart weight LDH, lactate dehydrogenase MDA, malondialdehyde Matrine Oxidative stress PPARs, peroxisomal proliferators-activated receptors ROS, reactive oxygen species SOD2, superoxide dismutase 2 T-caspase3, total-caspase3 TL, tibia length TUNEL, TdT-mediated dUTP nick end-labelling Top2, topoisomerase-II UCP2 UCP2, uncoupling protein 2 cTnT, cardiac isoform of Tropnin T
来 源:
DOI:10.1016/j.apsb.2019.03.003
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