背景:牙髓中的龋齿会导致牙髓组织的炎症和损伤。在牙髓发炎期间,各种炎症介质和生长因子被释放,包括IL-8、IL-10、TLR-2、VEGF和TGF-β通过NF-kB途径。在本研究中,通过给予蜂胶和氢氧化钙(Ca(OH)2)的组合,通过盖髓进行牙髓龋的治疗。预期该治疗刺激作为抗炎材料的修复性牙本质的形成以防止牙髓组织损伤。方法:将28只Wistar大鼠分为四组,在添加或不添加蜂胶的情况下用Ca(OH)2处理7或14天。免疫组化检测IL-8、IL-10、TLR-2、VEGF、四个治疗组中的TGF-β。结果:蜂胶和Ca(OH)2联合治疗7天,IL-10、IL-8、TLR-2、VEGF、与仅用Ca(OH)2处理的处理组相比,TGF-β显著增加。IL-10、TLR-2、TGF-β、用蜂胶和Ca(OH)2治疗14天的治疗组VEGF增加,而IL-8的表达明显下降。结论:蜂胶和Ca(OH)2的组合在盖髓治疗过程中具有功效,因为它具有抗菌和免疫调节特性。结果表明,通过增加IL-10、TGF-β的表达,能够刺激牙髓组织修复过程,VEGF,TLR-2和IL-8的表达下降。
Background: Caries in the dental pulp result in inflammation and damage to the pulp tissue. During inflammation of the pulp, various inflammatory mediators and growth factors are released, including IL-8, IL-10, TLR-2, VEGF and TGF-β through the NF-kB pathway. In the present study, therapy for pulpal caries was performed through pulp capping by giving a combination of propolis and calcium hydroxide (Ca(OH)2). This treatment was expected to stimulate the formation of reparative dentin as an anti-inflammatory material to prevent pulp tissue damage. Methods: 28 Wistar rats were divided into four groups and treated with Ca(OH)2 with or without the addition of propolis for either 7 or 14 days. Immunohistochemical examination was used to determine the expression of IL-8, IL-10, TLR-2, VEGF, TGF-β in the four treatment groups. Results: The group treated with a combination of propolis and Ca(OH)2 for 7 days showed that the expression of IL-10, IL-8, TLR-2, VEGF, TGF-β increased significantly compared to the treatment group treated with only Ca(OH)2. The expression of IL-10, TLR-2, TGF-β, VEGF increased in the treatment group treated with propolis and Ca(OH)2 for 14 days, while the expression of IL-8 in the decreased significantly. Conclusions: Administration of a combination of propolis and Ca(OH)2 has efficacy in the pulp capping treatment process because it has anti-bacterial and immunomodulatory properties. The results show that it is able to stimulate the process of pulp tissue repair through increased expression of IL-10, TGF-β, VEGF, TLR -2 and decreased expression of IL-8.