未经批准:Trigonellafoenum-graecumL.种子(TFG)在印度美食中用作香料。在印度传统医学中,TFG用于治疗糖尿病,血脂异常,肥胖,关节炎,癌症,消化系统疾病,和绝经后的情况。绝经后疾病的病理生理学涉及低度全身性炎症。本研究的目的是研究石油醚部分TFG提取物(PE-TFG)对炎症标志物的预防作用,和高脂饮食(HFD)喂养的去卵巢大鼠(OVX大鼠)的组织病理学变化。
未经证实:OVX雌性SpragueDawley大鼠用于研究。卵巢切除术后三周,将大鼠随机分为不同的组,并给予PE-TFG,阿托伐他汀,皂苷元,17β-雌二醇与HFD一起持续12周。假手术大鼠(S.OVX)用标准颗粒饮食喂养。在12周结束时,老鼠被处死,血液样本被用来估计血脂,葡萄糖,肝标志物,TNF-α,还有瘦素.肝脏,肾,和颈总动脉被分离用于测试氧化应激标志物,脂联素的mRNA表达,PPAR-γ,和组织病理学变化。
UNASSIGNED:给予PE-TFG显着降低(P<0.05)总胆固醇,LDL,肝标志物,瘦素,TNF-α和脂联素和PPAR-γ在HFD喂养OVX大鼠中的表达。Further,微观和宏观肝脂肪变性,炎症,肾小球肥大,肾脏中退化的小管,内膜增加,与S.OVX大鼠相比,PE-TFG给药后的颈总动脉中层厚度和病理变化不明显。
未经证实:PE-TFG在HFD喂养的OVX-大鼠中保护细胞炎症和代谢变化,因此可以作为预防剂在绝经后疾病中进一步探索。
UNASSIGNED: Trigonella foenum-graecum L. seeds (TFG) are used as spices in Indian cuisine. In Indian traditional medicine, TFG is used to treat diabetes, dyslipidemia, obesity, arthritis, cancer, digestive disorders, and postmenopausal conditions. Pathophysiology of postmenopausal diseases involves low-grade systemic inflammation. The purpose of this study is to investigate the prophylactic effect of petroleum ether fraction of TFG-extract (PE-TFG) on inflammatory markers, and histopathological changes in ovariectomized rats (OVX-rats) fed with a high-fat diet (HFD).
UNASSIGNED: OVX female Sprague Dawley rats were used for the study. Three weeks after ovariectomy, rats were randomized in different groups and administered PE-TFG, atorvastatin, diosgenin, 17β-estradiol for 12 weeks along with HFD. The sham-operated rats (S.OVX) were fed with a standard pellet diet. At the end of 12-weeks, rats were sacrificed, and blood samples were used to estimate lipid profile, glucose, hepatic markers, TNF-α, and leptin. Liver, kidney, and common carotid artery were isolated for testing oxidative stress markers, mRNA expression of adiponectin, PPAR-γ, and histopathological changes.
UNASSIGNED: Administration of PE-TFG significantly decreased (P < 0.05) total cholesterol, LDL, hepatic markers, leptin, TNF-α and improved mRNA expression of adiponectin and PPAR-γ in HFD-fed OVX-rats. Further, micro and macro hepatic steatosis, inflammation, glomerular hypertrophy, degenerated tubules in kidney, increased tunica intima, and media thickness of common carotid artery and the pathological changes were not significant upon PE-TFG administration compared to S.OVX-rats.
UNASSIGNED: PE-TFG protects cellular inflammation and metabolic alternations in HFD-fed OVX-rats and thus can be explored further in postmenopausal diseases as a prophylactic agent.