新城疫病毒(NDV)溶胞产物已被确定为用于肿瘤治疗的独特且有效的免疫刺激根。因此,本研究的目的是研究NDV溶胞产物瘤内给药对人乳头瘤病毒(HPV)相关的TC-1同源癌细胞移植的C57BL/6小鼠模型的免疫反应和肿瘤消退的影响。为了进一步研究抗肿瘤反应的潜在机制,使用乳酸脱氢酶(LDH)释放和MTT测定法测量脾细胞中的细胞溶解和淋巴细胞增殖反应,分别。在这方面,IL-10,IFN-γ,再刺激后使用ELISA测量IL-4。通过体内肿瘤回归分析评估免疫应答功效。结果显示,与病毒裂解物和肿瘤细胞裂解物的组合相比,用不同滴度的NDV裂解物的免疫显著减少肿瘤体积。此外,与肿瘤细胞裂解液相比,病毒裂解液可以显着提高细胞毒性T淋巴细胞的产生和淋巴细胞的增殖率。此外,我们的主要发现是,瘤周注射的NDV溶胞产物通过增加IL-4,IFN-γ的水平有效地诱导抗肿瘤免疫反应,和IL-10的减少。这些结果表明,这种治疗是一种特殊的,主动免疫机制刺激器,并可能被证明是一种有用的治疗子宫颈癌的治疗方法,值得进一步研究。
A Newcastle disease virus (NDV) oncolysate has been established as a unique and effective immune-stimulatory root for tumor treatment. Thus, the aim of the current study was to investigate the effects of intratumoral administration of NDV oncolysate on immune response and tumor regression of C57BL/6 mouse model of human papillomavirus (HPV) related transplanted with TC-1 syngeneic cancer cells. To further investigate the mechanism underlying the antitumor response, cytolytic and lymphocyte proliferation responses in splenocytes were measured using lactate dehydrogenase (LDH) release and MTT assays, respectively. In this regard, levels of IL-10, IFN-γ, and IL-4 were measured using ELISA after re-stimulation. The immune responses efficacy was evaluated by in vivo tumor regression assay. The results showed that immunization with the different titers of NDV lysate significantly reduced tumor volume in comparison with a combination of virus lysate and tumor cell lysate. Also, virus lysate could significantly enhance cytotoxic T lymphocyte production and lymphocyte proliferation rates versus tumor cell lysate. Also, our major findings are that the peritumorally injection of NDV oncolysate effectively induces antitumor immune responses through increased levels of IL-4, IFN-γ, and reduction of IL-10. These results indicate that this treatment is a specific, active immune mechanism stimulator, and may prove to be a useful therapeutic for a treatment against cervical cancers and merits further investigation.