■由于糖尿病(DM)的死亡率和发病率不断增加,这是我们这个时代最大的健康问题之一,许多治疗方式仍在尝试中。二甲双胍(MET)和体育锻炼(EXE)对糖尿病病理生理学的积极作用是众所周知的。在这项研究中,它的目的是在炎症的基础上详细说明MET和EXE的这些积极作用,凋亡机制,和内生素nesfatin-1(NES-1)合成。
■将27只2型DM(DM-2)雄性Wistar白化病大鼠分为4组,作为高脂肪饮食(HFD),MET,EXE,和MET+EXE组。研究的总持续时间为3个月。实验结束时,测量血糖和血脂。对心脏和主动脉组织进行组织病理学评估,并通过免疫组织化学评估凋亡标志物。通过酶联免疫吸附试验分析炎症标志物和NES-1水平。
■血浆葡萄糖,稳态模型评估-胰岛素抵抗(HOMA-IR),低密度脂蛋白(LDL)水平升高,HFD组高密度脂蛋白(HDL)水平显著下降。在治疗组中,葡萄糖,HOMA-IR,LDL,血浆中的NES-1水平,以及肿瘤坏死因子α(TNF-α),白细胞介素-1β(IL-1β),IL-6,caspase-3(Cas-3),Bcl-2相关X蛋白(Bax),组织中炎症的组织病理学发现减少。此外,血浆胰岛素增加,HDL,和组织B细胞淋巴瘤-2和水平。
■观察到DM-2模型中的MET和EXE处理减少了细胞损伤机制,例如炎症和凋亡。NES-1水平的降低被认为是这种抗炎作用的次要因素。总之,结果证明了EXE在降低DM-2和NES-1水平方面的有效性.需要进一步的研究来评估不同EXE模型和治疗持续时间的效果。
UNASSIGNED: Due to the increasing mortality and morbidity rates in diabetes mellitus (DM), which is one of the biggest health problems of our age, many treatment modalities are still being tried. The positive effects of metformin (MET) and physical exercise (EXE) on the pathophysiology of diabetes are well known. In this study, it was aimed to detail these positive effects of MET and EXE in combination on the basis of inflammation, apoptosis mechanisms, and endogen nesfatin-1 (NES-1) synthesis.
UNASSIGNED: Twenty-seven type 2 DM (DM-2) male Wistar Albino rats were divided into 4 groups, as the high-fat diet (HFD), MET, EXE, and MET+EXE groups. The total duration of the study was 3 months. At the end of the experiment, blood glucose and lipid profiles were measured. Histopathological evaluation was performed on the cardiac and aortic tissues and apoptotic markers were evaluated immunohistochemically. Inflammatory markers and NES-1 levels were analyzed by enzyme-linked immunosorbent assay.
UNASSIGNED: The plasma glucose, homeostatic model evaluation-insulin resistance (HOMA-IR), low-density lipoprotein (LDL) levels increased, and high-density lipoprotein (HDL) levels decreased significantly in the HFD group. In the treatment groups, the glucose, HOMA-IR, LDL, NES-1 levels in the plasma, as well as tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), IL-6, caspase-3 (Cas-3), Bcl-2-associated X protein (Bax), and histopathological findings of inflammation in tissues were decreased. Additionally, there was an increase in plasma insulin, HDL, and tissue B-cell lymphoma-2 and levels.
UNASSIGNED: It was observed that the MET and EXE treatments in the DM-2 model reduced cellular damage mechanisms such as inflammation and apoptosis. The decrease in NES-1 levels was thought to be secondary to this antiinflammatory effect. In conclusion, the results demonstrated the effectiveness of EXE in reducing DM-2 and the NES-1 levels. Further studies are needed to evaluate the effect in different EXE models and treatment durations.