关键词: Ankylosing spondylitis Irisin Pathological bone features Swimming exercise

Mesh : Animals Fibronectins / metabolism beta-Glucans / pharmacology administration & dosage Swimming / physiology Mice Male Physical Conditioning, Animal Humans Female Disease Models, Animal

来  源:   DOI:10.1016/j.lfs.2024.122894

Abstract:
This study assessed the therapeutic potential of swimming exercise in the curdlan-injected SKG mouse model and investigated the modulatory effects of irisin on inflammation. Curdlan-injected SKG were randomly assigned to either a home-cage group or a swimming group for 6 weeks. Changes in clinical arthritis scores and ankle thickness were measured weekly. Post-swimming program, mice were anesthetized for collection of vastus lateralis muscle and blood, which was followed by histological analysis, micro-CT imaging of the ankle joints, and the measurement of pro-inflammatory cytokines and irisin levels. Additionally, curdlan-injected SKG mice were intravenously injected with recombinant irisin protein and observed. Finally, serum levels of irisin in healthy control and ankylosing spondylitis (AS) patient groups were measured by ELISA. The swimming group of curdlan-injected SKG mice exhibited significant improvements in arthritis and enthesitis compared to the home-cage group. In particular, micro-CT and histological analyses revealed a notable reduction in pathological bone features in the swimming group compared to the home-cage group. Muscle endurance was also enhanced in the swimming group compared to the home-cage group, as determined by the wire-hanging test. Intriguingly, irisin levels not only were statistically increased in the swimming group but, also, TNF-α, IL-1β, and IL-6 levels were decreased. Additionally, injection of irisin protein slightly attenuated both arthritis and enthesitis in curdlan-injected SKG mice. Meanwhile, irisin serum levels were declined in AS patients. Overall, we found that swimming exercise attenuated pathological bone features in an AS animal model, potentially mediated by increased irisin serum levels with associated anti-inflammatory effects.
摘要:
这项研究评估了游泳运动在注射curdlan的SKG小鼠模型中的治疗潜力,并研究了irisin对炎症的调节作用。注射Curdlan的SKG被随机分配到家庭笼组或游泳组,为期6周。每周测量临床关节炎评分和踝关节厚度的变化。游泳后计划,将小鼠麻醉以收集股外侧肌和血液,随后进行组织学分析,踝关节的显微CT成像,以及促炎细胞因子和irisin水平的测量。此外,用重组irisin蛋白静脉注射注射curdlan的SKG小鼠并观察。最后,ELISA法检测健康对照组和强直性脊柱炎(AS)患者组血清irisin水平。与家庭笼组相比,注射了Curdlan的SKG小鼠的游泳组表现出关节炎和附着性炎的显着改善。特别是,Micro-CT和组织学分析显示,与家庭笼组相比,游泳组的病理性骨特征显着减少。与家庭笼组相比,游泳组的肌肉耐力也得到了增强,由挂线试验确定。有趣的是,游泳组的irisin水平不仅在统计学上增加,而且,还,TNF-α,IL-1β,IL-6水平降低。此外,注射irisin蛋白可略微减轻注射curdlan的SKG小鼠的关节炎和附着点炎。同时,AS患者血清irisin水平下降。总的来说,我们发现游泳运动减弱了AS动物模型的病理性骨特征,可能由与相关的抗炎作用增加的irisin血清水平介导。
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