foenum-graecumTrigonella,被称为胡芦巴,属于西亚野生豆科,欧洲,地中海,和亚洲;其成熟的种子含有大量生物活性物质,在食品工业和医药领域具有巨大潜力。在这项研究中,提取胡芦巴种子粘液(FSM),并通过X射线衍射表征其结构特性,核磁共振,和高效液相色谱法。然后,FSM作为抗微生物剂的适用性通过新型,活跃,含有羧甲基纤维素和迷迭香精油(REO)的可食用基于FSM的生物膜。在生物膜中加入REO会引起傅立叶变换红外光谱的特定变化,影响热降解行为。扫描电子显微镜和原子力显微镜形态图显示REO的均匀分布和负载膜中的更光滑表面。此外,溶解度测试表明,随着REO浓度从1重量%增加至3重量%,水溶解度降低。生物测定证明了负载REO的生物膜对金黄色葡萄球菌和大肠杆菌的抗微生物活性。最后,用基于FSM的解决方案浸涂整个苹果,以展示未来的可食用系统。载有REO的生物膜将苹果的保质期延长到30天,展示他们在可持续和活性涂料方面的潜力。
Trigonella foenum-graecum, known as fenugreek, belongs to the leguminous family of wild growth in Western Asia, Europe, the Mediterranean, and Asia; its ripe seeds contain a pool of bioactive substances with great potential in the food industry and medicine. In this study, fenugreek seed mucilage (FSM) was extracted and characterized in its structural properties by X-ray diffraction, nuclear magnetic resonance, and high-performance liquid chromatography. Then, the applicability of FSM as an antimicrobial agent was demonstrated via the development of novel, active, edible FSM-based biofilms containing carboxymethyl cellulose and rosemary essential oil (REO). Incorporating REO in the biofilms brought about specific changes in Fourier-transform infrared spectra, affecting thermal degradation behavior. Scanning electron microscopy and atomic force microscopy morphography showed an even distribution of REO and smoother surfaces in the loaded films. Besides, the solubility tests evidenced a reduction in water solubility with increasing REO concentration from 1 to 3 wt%. The biological assay evidenced the antimicrobial activity of REO-loaded biofilms against Staphylococcus aureus and Escherichia coli. Finally, whole apples were dip-coated with FSM-based solutions to showcase future edible systems. The REO-loaded biofilms extended the shelf life of apples to 30 days, demonstrating their potential for sustainable and active coatings.