Abstract：
Active bacterial nanocellulose (BNC) nanopapers containing Salvia officinalis essential oil (SEO) in free form and encapsulated with β-cyclodextrin (βCD) were prepared, and their effect on the shelf life extension of shrimp was investigated. The GC-MS analysis of the SEO indicated the presence of various active compounds such as Thujone (21.53 %), Ledol (12.51 %) and Eucalyptol (11.28 %) in the essential oil composition. The cytotoxicity of the SEO and SEO-βCD complexes in the L929 cell line was quite low. FTIR analysis revealed new interactions in the nanopapers containing SEO-βCD complexes. Microscopic images showed that SEO-βCD complexation improved the surface morphology of the BNC nanopapers, whereas free SEO had a negative effect. X-ray diffraction patterns of the nanopapers showed higher crystallinity of the SEO-βCD containing nanopapers than that of the SEO-incorporated nanopapers. Moreover, the addition of the SEO-βCD complex improved the thermal properties of the BNC nanopaper. Water contact angle analysis showed higher hydrophobicity of the samples containing free SEO than that of the other samples. Both SEO-βCD and free SEO increased the elongation at break and decreased the tensile strength of the nanopaper. The prepared active films showed a greater antimicrobial effect on L. monocytogenes than on E. coli. The results showed a higher antioxidant capacity of the free SEO-containing nanopapers (58-78 %). The desirable effects of the active nanopapers on shrimp preservation were demonstrated by the results obtained for the microbial load, pH, and volatile nitrogen content of the product. The results demonstrate the potential of the prepared BNC active nanopapers for use in active antioxidant/antimicrobial food packaging.
摘要：
制备了含有游离形式的丹参精油（SEO）并用β-环糊精（βCD）包封的活性细菌纳米纤维素（BNC）纳米纸，研究了它们对虾货架期延长的影响。SEO的GC-MS分析表明存在各种活性化合物，例如Thujone（21.53％），精油组合物中的Ledol（12.51％）和Eucalypol（11.28％）。SEO和SEO-βCD复合物在L929细胞系中的细胞毒性相当低。FTIR分析揭示了含有SEO-βCD复合物的纳米纸中的新相互作用。显微图像显示SEO-βCD复合改善了BNC纳米纸的表面形貌,而免费SEO有负面影响。纳米纸的X-射线衍射图显示含SEO-βCD的纳米纸的结晶度高于掺入SEO的纳米纸的结晶度。此外，SEO-βCD复合物的加入改善了BNC纳米纸的热性能。水接触角分析显示含有游离SEO的样品的疏水性高于其他样品。SEO-βCD和游离SEO都增加了断裂伸长率，并降低了纳米纸的拉伸强度。所制备的活性膜对单核细胞增生李斯特菌显示出比对大肠杆菌更大的抗微生物作用。结果显示游离的含SEO的纳米纸具有更高的抗氧化能力(58-78%)。通过微生物负载获得的结果证明了活性纳米纸对虾保存的理想效果，pH值,和产品的挥发性氮含量。结果证明了所制备的BNC活性纳米纸片用于活性抗氧化剂/抗微生物食品包装的潜力。
