背景和目的:高泌乳素血症,作为一些抗精神病药物的潜在副作用,与骨密度降低和骨折风险增加有关。这项研究调查了补充钙和维生素D是否影响十二指肠中的催乳素受体(Prlr)基因表达,椎骨,舒必利诱导的高催乳素血症雌性大鼠的肾脏。材料和方法:将21周龄雌性Wistar大鼠分为三组:S组由10只大鼠组成,每天两次接受舒必利注射(10mg/kg),持续6周;D组(10只)在最后3周每天补充50mg钙和500IU维生素D以及舒必利;C组由7只年龄匹配的未出生大鼠组成,作为对照组。实时PCR用于评估十二指肠中Prlr基因的表达,椎骨,还有肾脏.结果:S组,与C组相比,Prlr基因表达在十二指肠中显著降低(p<0.01),但在椎骨和肾脏中升高。D组表现出在十二指肠中Prlr表达显著增加(p<0.01),同时在椎骨和肾脏中表达增加。结论:在舒必利引起的高催乳素血症中,十二指肠中Prlr基因表达降低可能导致肠道钙吸收降低。因此,催乳素可以从骨骼系统中吸收钙以维持钙平衡,椎骨中Prlr基因表达增加。然而,舒必利诱导的高催乳素血症中补充维生素D显着增强十二指肠中的Prlr基因表达,可能改善肠道钙吸收和减轻对骨骼健康的不利影响。
Background and Objectives: Hyperprolactinemia, as a potential side-effect of some antipsychotic medications, is associated with decreased bone density and an increased risk of fractures. This study investigates whether calcium and vitamin D supplementation affects prolactin receptor (Prlr) gene expression in the duodenum, vertebrae, and kidneys of female rats with
sulpiride-induced hyperprolactinemia. Materials and Methods: Twenty-one-week-old female Wistar rats were assigned to three groups: Group S consisted of ten rats who received
sulpiride injections (10 mg/kg) twice daily for 6 weeks; Group D (10 rats) received daily supplementation of 50 mg calcium and 500 IU vitamin D along with
sulpiride for the last 3 weeks; and Group C consisting of seven age-matched nulliparous rats serving as a control group. Real-time PCR was used to assess Prlr gene expression in the duodenum, vertebrae, and kidneys. Results: In Group S, Prlr gene expression was notably decreased in the duodenum (p < 0.01) but elevated in the vertebrae and kidneys compared to Group C. Conversely, Group D exhibited significantly increased Prlr expression in the duodenum (p < 0.01) alongside elevated expression in the vertebrae and kidneys. Conclusions: In
sulpiride-induced hyperprolactinemia, decreased Prlr gene expression in the duodenum may lead to reduced intestinal calcium absorption. Consequently, prolactin may draw calcium from the skeletal system to maintain calcium balance, facilitated by increased Prlr gene expression in the vertebrae. However, vitamin D supplementation in
sulpiride-induced hyperprolactinemia notably enhances Prlr gene expression in the duodenum, potentially ameliorating intestinal calcium absorption and mitigating adverse effects on bone health.