衣酯是一种有前途的平台化学品,具有广泛的适用性,包括聚(甲基丙烯酸甲酯)的合成。大多数关于微生物衣康酸酯生产的研究都需要使用基于作物的原料,由于其有限的供应而施加了限制。棕色大型藻类由于其高生物质生产率和碳水化合物含量以及对大规模生产的适应性而最近作为下一代生物质受到关注。因此,大型藻类用于衣康酸酯生产值得探索。在这项研究中,使用工程弧菌sp证明了从棕色大型藻类直接生产衣康酸酯。dhg,已成为棕色大型藻类生物精炼厂的有效平台宿主。具体来说,为了提高产量,来自土曲霉的顺式-乌头酸脱羧酶(Cad)被异源表达,异柠檬酸脱氢酶(icd)被删除。值得注意的是,由此产生的应变,VIC,从藻酸盐和甘露醇的混合物(每种10g/L)和40g/L的生日本糖精(S.粳稻),分别。总的来说,这项研究强调了棕色大型藻类作为原料的实用性,以及弧菌。dhg作为提高衣康酸生物生产的平台菌株。
Itaconate is a promising platform chemical with broad applicability, including the synthesis of poly(methyl methacrylate). Most studies on microbial itaconate production entail the use of crop-based feedstock, which imposes constraints due to its limited supply. Brown macroalgae have recently gained attention as next-generation biomass owing to their high biomass productivity and carbohydrate content and amenability to mass production. Therefore, the use of macroalgae for itaconate production warrants exploration. In this study, the direct production of itaconate from brown macroalgae was demonstrated using engineered Vibrio sp. dhg, which has emerged as an efficient platform host for brown macroalgal biorefineries. Specifically, to enhance production, cis-aconitate decarboxylase (Cad) from Aspergillus terreus was heterologously expressed and isocitrate dehydrogenase (icd) was deleted. Notably, the resulting strain, VIC, achieved itaconate titers of 2.5 and 1.5 g/L from a mixture of alginate and mannitol (10 g/L of each) and 40 g/L of raw Saccharina japonica (S. japonica), respectively. Overall, this study highlights the utility of brown macroalgae as feedstock, as well as that of Vibrio sp. dhg as a platform strain for improving itaconate bioproduction.