为了评估循环水系统中不同底物对乳晕巴比伦菌免疫反应和抗氧化能力的影响,我们对三种底物环境(sand-S组,陶瓷颗粒-C组,和PVC育巢-P组)。转录组结果表明,S组和P组表现出最高数量的差异表达基因(DEGs),总共2218个DEG,包括928个上调的DEG和1290个下调的DEG。C组和P组共有1055个DEG,316个上调和739个下调的DEG。C组和S组的DEG最少,总共521个,包括303个上调的DEG和218个下调的DEG。GO富集分析表明,在S-P组,如催化活性,膜部分,细胞过程富集了287、262和180个DEG,分别。在CvsP组中,绑定,细胞过程,细胞部分富集了146、135和127个DEG,分别。在CvsS组中,催化活性,膜部分,代谢过程富集了90、83和59个DEGs,分别。Kegg富集分析显示S与P组的免疫相关途径发生了显着变化,包括溶酶体,吞噬体,和白细胞跨内皮迁移,有30、13和10个丰富的DEG,分别。在CvsP组中,吞噬体,药物代谢-其他酶,和N-聚糖生物合成显示免疫相关途径的显著变化,有9个、6个和4个丰富的DEG,分别。在CvsS组中,溶酶体,PPAR信号通路,和脂肪酸降解表现出免疫相关途径的显著变化,有8、4和3个丰富的DEG,分别。关于抗氧化能力,S组总T-AOC明显高于其他实验组,而CAT,SOD,POD,AKP低于C组和P组。沙组的ACP水平与P组无明显差异,但明显低于C组。总之,底物环境显著影响areolata中免疫相关基因和关键抗氧化酶的活性。
To assess the impact of different
substrates in a recirculating water system on the immune response and antioxidant capacity of Babylonia areolata, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand-S group, ceramic granules-C group, and PVC breeding nest-P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism-other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in B. areolata.