Strigolactone(SL)是调节芽分枝的植物激素。此外,SL充当刺激根寄生杂草发芽的化合物,如Strigaspp.和Orobanchespp.,对全球农业造成重大损害。因此,SL激动剂有可能诱导自杀发芽,从而减少土壤中根寄生杂草的种子库。特别是,苯氧基呋喃酮SL激动剂,被称为debranones,在水稻和Strigahermonthica中表现出类似SL的活性。然而,人们对它们对Orobanchespp的影响知之甚少。在这项研究中,我们评估了debranones对Orobancheminor的发芽诱导活性。结构-活性关系的分析表明,在2,4-或2,6-位置具有吸电子取代基的debranones强烈诱导了Orobancheminor的萌发。最后,生物测定表明,5-(2-氟-4-硝基苯氧基)-3-甲基呋喃-2(5H)-酮(测试化合物61)诱导萌发的程度与GR24(一种众所周知的合成SL)相当或甚至更强。总之,我们的数据使我们能够推断这种增强的活性是由于SLs受体对化合物61的识别,KAI2d,在Orobanche小调.
Strigolactones (SLs) are plant hormones that regulate shoot branching. In addition, SLs act as compounds that stimulate the germination of root parasitic weeds, such as Striga spp. and Orobanche spp., which cause significant damage to agriculture worldwide. Thus, SL agonists have the potential to induce suicidal germination, thereby reducing the seed banks of root parasitic weeds in the soil. Particularly, phenoxyfuranone-type SL agonists, known as debranones, exhibit SL-like activity in rice and Striga hermonthica. However, little is known about their effects on Orobanche spp. In this study, we evaluated the germination-inducing activity of debranones against Orobanche minor. Analysis of structure-activity relationships revealed that debranones with electron-withdrawing substituents at the 2,4- or 2,6-position strongly induced the germination of Orobanche minor. Lastly, biological assays indicated that 5-(2-fluoro-4-nitrophenoxy)-3-methylfuran-2(5H)-one (test compound 61) induced germination to a comparable or even stronger extent than GR24, a well-known synthetic SL. Altogether, our data allowed us to infer that this enhanced activity was due to the recognition of compound 61 by the SLs receptor, KAI 2d, in Orobanche minor.