■蝙蝠,以及它们的外寄生虫,拥有广泛多样的共生和潜在的致病菌。尽管蝙蝠种类繁多(181种),很少有研究旨在调查巴西翼翅目和相关外寄生虫的细菌微生物组。这项研究旨在表征南马托格罗索州的非食血蝙蝠和相关的Streblidae蝇以及Macronyssidae和Spinturnicidae螨的细菌微生物组,巴西中西部。
■从30只蝙蝠中收集了口腔和直肠拭子(Artibeuslituratus[n=13],Artibeusplanirostris[n=9],弗氏Eptesicusfurinalis[n=5],Carolliaperspicillata[n=2],和Platyrhinuslineatus[n=1])。此外,从捕获的蝙蝠中总共收集了58只螨虫(15只Macronyssidae和43只Spinturnicidae)和48只Streblidae蝙蝠蝇。经过DNA提取和纯化,用宏基因组测序分析每个样本的细菌组成。
■口服和直肠蝙蝠拭子样品的微生物组组成表明,γ变形杆菌是最丰富的细菌类别。螺旋虫,Wolbachia和Bartonella代表了Streblidae蝇中最丰富的属。虽然Wolbachia(α变形杆菌)是脊柱科中发现的最丰富的属,在Macronyssidae螨虫中发现了高丰度的Arsenophonus(γ变形杆菌)。除了在类和属分类水平上表征每个样品的微生物组之外,我们确定了能够在口腔(链球菌和无性体)和直肠拭子(肠杆菌,克雷伯菌属,埃希氏菌,肠球菌,链球菌),Macronyssidae(无性体,巴尔通体,埃里希氏菌)和脊柱科(无性体,Bartonella)螨虫以及Streblidae蝇(螺旋体,巴尔通体)。
除了扩大对来自巴西的非食血蝙蝠和Streblidae苍蝇的细菌微生物组的了解之外,目前的工作表明,第一次,与蝙蝠相关的Macronyssidae和Spinturnicidae螨的细菌群落。
UNASSIGNED: Bats, along with their ectoparasites, harbor a wide diversity of symbiotic and potential pathogenic bacteria. Despite the enormous diversity of bats (181 species), few studies aimed to investigate the bacterial microbiome of Brazilian chiropterans and associated ectoparasites. This study aimed to characterize the bacterial microbiome of non-hematophagous bats and associated
Streblidae flies and Macronyssidae and Spinturnicidae mites in the state of Mato Grosso do Sul, midwestern Brazil.
UNASSIGNED: Oral and rectal swabs were collected from 30 bats (Artibeus lituratus [n = 13], Artibeus planirostris [n = 9], Eptesicus furinalis [n = 5], Carollia perspicillata [n = 2], and Platyrrhinus lineatus [n = 1]). In addition, a total of 58 mites (15 Macronyssidae and 43 Spinturnicidae) and 48
Streblidae bat flies were collected from the captured bats. After DNA extraction and purification, each sample\'s bacterial composition was analyzed with metagenomic sequencing.
UNASSIGNED: The microbiome composition of both oral and rectal bat swab samples showed that Gammaproteobacteria was the most abundant bacterial class. Spiroplasma, Wolbachia and Bartonella represented the most abundant genera in
Streblidae flies. While Wolbachia (Alphaproteobacteria) was the most abundant genus found in Spinturnicidae, Arsenophonus (Gammaproteobacteria) was found in high abundance in Macronyssidae mites. In addition to characterizing the microbiome of each sample at the class and genus taxonomic levels, we identified medically significant bacteria able to infect both animals and humans in oral (Streptococcus and Anaplasma) and rectal swabs (Enterobacter, Klebsiella, Escherichia, Enterococcus, Streptococcus), Macronyssidae (Anaplasma, Bartonella, Ehrlichia) and Spinturnicidae (Anaplasma, Bartonella) mites as well as
Streblidae flies (Spiroplasma, Bartonella).
UNASSIGNED: Besides expanding the knowledge on the bacterial microbiome of non-hematophagous bats and
Streblidae flies from Brazil, the present work showed, for the first time, the bacterial community of bat-associated Macronyssidae and Spinturnicidae mites.